CD11c antibody - HS-375 013

CD11c is a fibrinogen receptor involved in cell-cell interaction during inflammation

Rabbit polyclonal purified antibody

Cat. No.: HS-375 013

Amount: 50 µg

Price: $375.00

Cat. No. HS-375 013	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 2000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues surrounding AA 1010 of rat CD11c (UniProt Id: D3ZWZ1)
Reactivity	Reacts with: mouse (Q9QXH4), rat (D3ZWZ1). Other species not tested yet.
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is required. IHC-P: Antigen retrieval with Tris-EDTA buffer pH 9 is recommended for chromogenic detection.
Data sheet	hs-375_013.pdf

Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

Cat. No.: HS-375 013

Amount: 50 µg

Price: $375.00

Immunofluorescence image of a mouse spleen section stained for CD11c

IHC

IHC-P

Protocols

IHC

IHC-P