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IBA1 antibody - HS-234 013 K.O.

IBA1 is a marker for macrophages and microglia
Rabbit polyclonal purified antibody
Cat. No.: HS-234 013
Amount: 200 µl
Price: $380.00
Cat. No. HS-234 013 200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 200 gallery  
IHC-P: 1 : 100 gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse IBA1 (UniProt Id: Q9EQW9)
Reactivity Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
Specificity K.O. validated
Data sheet hs-234_013.pdf
Important information
This product belongs to the HistoSure product line of antibodies developed for and extensively tested in FFPE tissues.
For more information please visit our HistoSure brand website.

References for IBA1 - HS-234 013

Anticonvulsant Action and Long-Term Effects of Chronic Cannabidiol Treatment in the Rat Pentylenetetrazole-Kindling Model of Epilepsy.
Gáll Z, Kelemen K, Tolokán A, Zolcseak I, Sável I, Bod R, Ferencz E, Vancea S, Urkon M, Kolcsár M
Biomedicines (2022) 108: . HS-234 013 IHC; tested species: mouse
Cat. No.: HS-234 013
Amount: 200 µl
Price: $380.00
Anticonvulsant Action and Long-Term Effects of Chronic Cannabidiol Treatment in the Rat Pentylenetetrazole-Kindling Model of Epilepsy.
Gáll Z, Kelemen K, Tolokán A, Zolcseak I, Sável I, Bod R, Ferencz E, Vancea S, Urkon M, Kolcsár M
Biomedicines (2022) 108: . HS-234 013 IHC; tested species: mouse
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).