Synaptic Systems - Vimentin

Cat. No.: HS-172 011

Amount: 100 µg

Price: $420.00

Cat. No. HS-172 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	V9
Subtype	IgG1 (κ light chain)
Immunogen	Full-length pig Vimentin recombinant protein (UniProt Id: P02543)
Reactivity	Reacts with: human (P08670), rat (P31000). No signal: mouse (P20152). Other species not tested yet.
Data sheet	Datasheet hs-172_011

References for Vimentin - HS-172 011

Precise epitope determination of the anti-vimentin monoclonal antibody V9.
Tomiyama L, Kamino H, Fukamachi H, Urano T
Molecular medicine reports (2017) 164: 3917-3921. HS-172 011 WB; tested species: human,mouse

Angiotensin II stimulates vimentin phosphorylation via a Ca2+-dependent, protein kinase C-independent mechanism in cultured vascular smooth muscle cells.
Tsuda T, Griendling KK, Alexander RW
The Journal of biological chemistry (1988) 26336: 19758-63. HS-172 011 WB; tested species: rat

Evidence for a hepatocellular lineage in a combined hepatocellular-cholangiocarcinoma of transitional type.
Fisher HP, Doppl W, Osborn M, Altmannsberger M
Virchows Archiv. B, Cell pathology including molecular pathology (1988) 562: 71-6. HS-172 011 IHC; tested species: human

Ubiquitin and microtubule-associated protein tau immunoreactivity each define distinct structures with differing distributions and solubility properties in Alzheimer brain.
Shaw G, Chau V
Proceedings of the National Academy of Sciences of the United States of America (1988) 858: 2854-8. HS-172 011 IHC; tested species: human

Coexpression of keratin and vimentin in damaged and regenerating tubular epithelia of the kidney.
Gröne HJ, Weber K, Gröne E, Helmchen U, Osborn M
The American journal of pathology (1987) 1291: 1-8. HS-172 011 IHC, EM; tested species: human

Vimentin is preferentially expressed in high-grade ductal and medullary, but not in lobular breast carcinomas.
Domagala W, Woźniak L, Lasota J, Weber K, Osborn M
The American journal of pathology (1990) 1375: 1059-64. HS-172 011 IHC-P (FFPE); tested species: human

Vimentin expression appears to be associated with poor prognosis in node-negative ductal NOS breast carcinomas.
Domagala W, Lasota J, Dukowicz A, Markiewski M, Striker G, Weber K, Osborn M
The American journal of pathology (1990) 1376: 1299-304. HS-172 011 IHC-P (FFPE); tested species: human

Coexpression of cytokeratins and vimentin in common epithelial tumours of the ovary: an immunocytochemical study of eighty-three cases.
Viale G, Gambacorta M, Dell'Orto P, Coggi G
Virchows Archiv. A, Pathological anatomy and histopathology (1988) 4132: 91-101. HS-172 011 IHC-P (FFPE); tested species: human

Alveolar soft part sarcoma. An immunohistochemical, cytologic and electron-microscopic study and a quantitative DNA analysis.
Persson S, Willems JS, Kindblom LG, Angervall L
Virchows Archiv. A, Pathological anatomy and histopathology (1988) 4126: 499-513. HS-172 011 IHC-P (FFPE); tested species: human

Cat. No.: HS-172 011

Amount: 100 µg

Price: $420.00

Vimentin (red) and Synaptopodin (green) staining in glomeruli of rat kidney

WB
(1 img)

ICC
(1 img)

IHC
(3 img)

IHC-P (FFPE)
(6 img)

Background

Vimentin belongs to the family of intermediate filaments that can be subdivided into six major groups based on sequence similarity. Vimentin belongs to the type III category and is the predominant subunit protein of intermediate filaments in tissues of mesenchymal origin.
Like other intermediate filaments it plays a role in the cytoskeletal organization and maintenance of cell shape and morphology.

1 ) Styers ML et al., J. Cell. Sci. (2006)
2 ) Esue O et al., J. Biol. Chem. (2006)
3 ) Miyaguchi K et al., J. Struct. Biol. (1997)
4 ) Chiu FC et al., J. Neurochem. (1981)
5 ) Browning ET et al., J. Cell Biol. (1981)
6 ) Gard DL et al., Cell (1980)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Vimentin antibody - HS-172 011

References for Vimentin - HS-172 011