Synaptic Systems

Cat. No.: 554 003

Amount: 50 µg

Price: $380.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

KLRG1+ (red) FoxP3+ (green) regulatory T-cells (Tregs) in the mouse spleen

WB
(1 img)

IHC
(1 img)

IHC-P (FFPE)
(5 img)

Background

Killer cell lectin-like receptor G1 (KLRG1) is an inhibitory lectin-like type II transmembrane glycoprotein receptor expressed on the surface of natural killer (NK) cells and distinct subsets of T-cells, where it functions as an immune checkpoint. KLRG1 is primarily expressed on mature immune cells, while naïve cells show little to no expression (1). In human T-cells, KLRG1 expression increases markedly with age and cellular differentiation, with the highest levels observed in memory T-cells and highly differentiated terminal effector cells (2).
KLRG1 contributes to immune regulation through its interaction with cadherins, including E-, N-, and R-cadherin, which are key mediators of cell–cell adhesion (3). KLRG1 binding to cadherins raises NK and T-cell activation thresholds, limiting NK cytotoxicity and protecting cadherin-expressing tissues. Beyond its role in effector T cells and NK cells, KLRG1 has also been investigated in group 2 innate lymphoid cells (ILC2s) in the lung and skin (4,5). Importantly, KLRG1 can bind tumor-derived E-cadherin (6), highlighting its potential as a therapeutic target in cancer immunotherapy (7).

1 ) Zhang Y et al., Cell Commun Signal (2024)
2 ) Henson SM et al., Age (Dordr) (2009)
3 ) Borys SM et al., Front Immunol (2022)
4 ) Can UI et al., Cell Rep (2025)
5 ) Jia H et al., Front Immunol (2023)
6 ) Myers JA et al., J Immunol (2026)
7 ) Jones TA et al., J Immunother Cancer (2026)

Protocols

WB

IHC

IHC-P (FFPE)

Cat. No. 554 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) gallery IHC-P (FFPE): 1 : 1000 up to 1 : 4000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of mouse KLRG1 (UniProt Id: O88713)
Reactivity	Reacts with: mouse (O88713). Other species not tested yet.
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 is required.
Data sheet	Data Sheet 554_003 Safety Data Sheet (SDS)

KLRG1 antibody - 554 003