Synaptic Systems

Cat. No.: 546 008

Amount: 100 µg

Price: $420.00

Cat. No. 546 008	100 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 2000 (ECL detection) gallery ICC: 1 : 500 up to 1 : 2000 gallery IHC: 1 : 500 up to 1 : 2000 gallery IHC-P (FFPE): 1 : 400 gallery iDISCO: external data (see remarks) gallery ELISA: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. iDISCO is a fast and cost-effective sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. The clearing is carried out by benzyl-ether treatment. For additional experimental details, please refer to the „Remarks“ section or visit https://idisco.info. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section.
Clone	ASF.15241
Subtype	IgG1 (κ light chain)
Reactivity	Reacts with: mouse (Q8BGD7), rat (Q8CJH6), human (Q8IUM7). Other species not tested yet.
Remarks	This antibody was developed by Activity Signaling, San Diego, USA. iDISCO: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	Datasheet 546_008

References for Npas4 - 546 008

Biphasic Npas4 expression promotes inhibitory plasticity and suppression of fear memory consolidation in mice.
Brito DVC, Kupke J, Sokolov R, Cambridge S, Both M, Bengtson CP, Rozov A, Oliveira AMM
Molecular psychiatry (2024) : . 546 008 WB; tested species: mouse

Functionally distinct NPAS4-expressing somatostatin interneuron ensembles critical for motor skill learning.
Yang J, Serrano P, Yin X, Sun X, Lin Y, Chen SX
Neuron (2022) 11020: 3339-3355.e8. 546 008 WB, IHC; tested species: mouse

Profound downregulation of neural transcription factor Npas4 and Nr4a family in fetal mice neurons infected with Zika virus.
Alpuche-Lazcano SP, Saliba J, Costa VV, Campolina-Silva GH, Marim FM, Ribeiro LS, Blank V, Mouland AJ, Teixeira MM, Gatignol A
PLoS neglected tropical diseases (2021) 155: e0009425. 546 008 WB; tested species: mouse

Patient-derived anti-NMDAR antibody disinhibits cortical neuronal networks through dysfunction of inhibitory neuron output.
Andrzejak E, Rabinovitch E, Kreye J, Prüss H, Rosenmund C, Ziv NE, Garner CC, Ackermann F
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) : . 546 008 ICC; tested species: mouse

Npas4-mediated dopaminergic regulation of safety memory consolidation.
Ko B, Yoo JY, Yoo T, Choi W, Dogan R, Sung K, Um D, Lee SB, Kim HJ, Lee S, Beak ST, et al.
Cell reports (2023) 427: 112678. 546 008 IHC; tested species: mouse

Moderate effect of early-life experience on dentate gyrus function.
Rukundo P, Feng T, Pham V, Pieraut S
Molecular brain (2022) 151: 92. 546 008 IHC; tested species: mouse

Elimination of Calm1 long 3' UTR mRNA isoform by CRISPR-Cas9 gene editing impairs dorsal root ganglion development and hippocampal neuron activation in mice.
Bae B, Gruner HN, Lynch M, Feng T, So K, Oliver D, Mastick GS, Yan W, Pieraut S, Miura P
RNA (New York, N.Y.) (2020) : . 546 008 IHC; tested species: mouse

Cat. No.: 546 008

Amount: 100 µg

Price: $420.00

NPAS4 and c-Fos staining of mouse visual cortex after insect hunting

Moderate effect of early-life experience on dentate gyrus function.
Rukundo P, Feng T, Pham V, Pieraut S
Molecular brain (2022) 151: 92. 546 008 IHC; tested species: mouse

WB
(3 img)

ICC
(2 img)

IHC
(4 img)

IHC-P (FFPE)
(2 img)

iDISCO
(1 img)

Background

The neuronal PAS domain protein 4 (Npas4) is a neuron specific transcription factor that belongs to the inducible immediate‑early genes and plays a key role in controlling activity dependent inhibitory synapse formation (1, 2). It has been originally identified as an upregulated transcript in ischemic brain tissue (3).
Npas4 is activated within minutes by Ca²⁺ influx mediated neuronal depolarization and peaks after 1–2 h (1, 4). Unlike the classic activity markers c‑Fos, Arc or Egr1, which can be driven by a variety of signaling pathways like cAMP, neurotrophic factors or neuromodulators, Npas4 expression is selectively triggered by neuronal activity (1, 5). This strict coupling to synaptic activity gives Npas4 a higher fidelity as a read‑out of recent neuronal activity (1). The deletion of Npas4 leads to an impairment of neuronal plasticity, underlining its importance for the nervous system to adapt to sensory input (5, 6).

1 ) Lin Y et al., Nature (2008)
2 ) Bloodgood BL et al., Nature (2013)
3 ) Shamloo M et al., Eur J Neurosci (2006)
4 ) Bersten DC et al., PLoS One (2014)
5 ) Ramamoorthi K et al., Science (2011)
6 ) Ploski JE et al., PLoS One (2011)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Npas4 antibody - 546 008

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References for Npas4 - 546 008