Synaptic Systems

Cat. No.: 545 003

Amount: 50 µg

Price: $380.00

Immunostaining of a human melanoma section using IFI16 to visualize a component of the inflammasome.

WB
(1 img)

IHC-P (FFPE)
(5 img)

Background

Interferon gamma inducible protein 16 (IFI16) is a member of the PYHIN (pyrin and HIN domain–containing) protein family and functions as a critical mediator of innate immune sensing and inflammatory signaling. IFI16 acts as a nuclear and cytoplasmic DNA sensor capable of recognizing viral and aberrant self-DNA, thereby initiating downstream signaling through pathways including STING–TBK1–IRF3 to promote type I interferon and pro-inflammatory cytokine production (1). In addition to its role in interferon signaling, IFI16 participates in inflammasome activation (2), transcriptional regulation, and cell cycle control, underscoring its multifunctional nature in immune defence and cellular homeostasis. Dysregulation of IFI16 expression or signaling has been implicated in diverse pathological conditions, including viral infections, autoimmune diseases such as systemic lupus erythematosus and Sjögren syndrome, chronic inflammatory disorders, and cancer (3,4). Notably, IFI16 exhibits context-dependent roles in tumor biology, functioning as either a tumor suppressor or a promoter of inflammation-driven tumorigenesis depending on cellular and microenvironmental factors.

1 ) Unterholzner L et al., Nat Immunol (2010)
2 ) Kerur N et al., Cell Host Microbe (2011)
3 ) Morrone SR et al., Proc Natl Acad Sci U S A (2014)
4 ) Zhang F et al., Front Immunol (2020)

Protocols

WB

IHC-P (FFPE)

Other products for this target

Cat. No. 545 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues near the amino terminus of human IFI16 (UniProt Id: Q16666)
Reactivity	Reacts with: human (Q16666). Other species not tested yet.
Data sheet	Datasheet 545_003

IFI16 antibody N-terminal - 545 003