Synaptic Systems

Cat. No.: 541 003

Amount: 50 µg

Price: $380.00

Mfsd2a-positive blood vessels in a mouse brain section

IHC-Fr

Background

Major facilitator superfamily domain-containing protein 2A (Mfsd2a) is a transmembrane protein that functions as a sodium-dependent transporter of lysophosphatidylcholine (LPC)–bound fatty acids, including the essential omega-3 fatty acid docosahexaenoic acid (DHA) (1). It is selectively expressed in endothelial cells of the central nervous system (CNS) vasculature and is a key regulator of blood–brain barrier (BBB) integrity (2). Mfsd2a maintains BBB function by suppressing caveolae-mediated transcytosis in brain endothelial cells, thereby limiting vesicular transport across the endothelium (2,3). Reduced expression of Mfsd2a has been associated with BBB disruption in several neurological conditions, including cerebral ischemia, hemorrhage, and chronic cerebral hypoperfusion (3,4).
In addition to its role at the BBB, Mfsd2a is expressed in other tissues where it contributes to lipid transport and metabolic processes. For instance, Mfsd2a has been implicated in lipid metabolism in the liver and in pulmonary surfactant homeostasis in the lung (5,6). Altered Mfsd2a expression has also been associated with cancer. Reduced Mfsd2a levels have been reported in non-small cell lung cancer and are linked to altered cell cycle regulation and cell–matrix interactions, suggesting a tumor suppressor role (7). Furthermore, downregulation of Mfsd2a in tumor-associated brain vasculature contributes to BBB disruption and altered lipid metabolism in brain metastases (8).

1 ) Nguyen LN et al., Nature (2014)
2 ) Moore CS et al., Am J Pathol (2012)
3 ) Andreone BJ et al., Neuron (2017)
4 ) Qu C et al., Front Neurosci (2020)
5 ) Pu W et al., Nat Commun (2016)
6 ) Wong BH et al., J Biol Chem (2022)
7 ) Spinola M et al., Mol Cancer (2010)
8 ) Tiwary S et al., Sci Rep (2018)

Protocols

IHC-Fr

Cat. No. 541 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: not tested yet IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues surrounding AA 20 of mouse Mfsd2a (UniProt Id: Q9DA75)
Remarks	IHC-Fr: Methanol fixation is recommended.
Data sheet	Datasheet 541_003

Mfsd2a antibody - 541 003