Synaptic Systems

Cat. No.: 528 017

Amount: 100 µg

Price: $420.00

Ly6G staining in a mouse lymph node section identifies neutrophils

ICC
(1 img)

IHC
(1 img)

IHC-P (FFPE)
(6 img)

Background

Ly6G is a member of the lymphocyte antigen 6 (Ly6) family, characterized by a conserved LU domain and lineage-specific expression on the surface of leukocytes (1). In mice, Ly6G is a small (~ 25 kD) protein that is anchored to the cell membrane via a C-terminal GPI anchor and serves as a functional homolog of human CD177 (1,2). Unlike Ly6C, which is expressed on a broader range of myeloid cells, Ly6G expression is largely restricted to neutrophils and granulocytic myeloid derived suppressor cells (MDSCs) in mice. Based on the differential expression of Ly6G and Ly6C, murine MDSCs can be subdivided into granulocytic/neutrophilic CD11b+ Gr-1+ Ly6G+ MDSCs, which express low levels of Ly6C and CD11b+ Gr-1+ Ly6C+ monocytic MDSCs, which express low levels of Ly6G (2). Ly6G is believed to play an important role in promoting neutrophil migration to sites of infection, thereby contributing to an effective immune response (3).

1 ) Lee PY et al., J Leukoc Biol (2013)
2 ) Höchst B et al., PLoS One (2015)
3 ) Kleinholz CL et al., Sci Rep (2021)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Cat. No. 528 017	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P (FFPE): 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-1B11
Subtype	IgG2a (κ light chain)
Immunogen	Recombinant protein corresponding to residues near the amino terminus of mouse Ly6G (UniProt Id: P35461)
Reactivity	Reacts with: mouse (P35461). Other species not tested yet.
Specificity	Specific for mouse Ly6G; no cross-reactivity with mouse Ly6C
Remarks	ICC: 4% formaldehyde/PFA fixation is recommended. IHC: Antigen retrieval with citrate buffer pH 6 is required.
Data sheet	Datasheet 528_017

Ly6G antibody - 528 017