Synaptic Systems

Cat. No.: 509 011

Amount: 100 µg

Price: $420.00

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Indirect immunostaining of a mouse hippocampus section with Prox1 (red), SATB2 (green) and PCP4 (blue)

ICC
(2 img)

IHC
(4 img)

IHC-P (FFPE)
(2 img)

Background

The prospero-related homeobox transcription factor 1, Prox1, is important for the embryonic development of various organs (1) including the eye, liver, pancreas, lymphatic system, heart and the central nervous system (CNS) (2). It is a regulator of cell division with impact on e.g. neurogenesis and cancer development and progression. In the CNS, the expression is not restricted to the embryonic development and to precursor cells. Prox1 is a well-known marker for granule cells of the dentate gyrus (DG) during embryonic development, adult neurogenesis but also for mature granule cells in the adult brain (3). Weaker expression can also be detected outside the DG of the adult brain. Prox1 was shown to be expressed in oligodendrocyte progenitors, e.g. in the subventricular zone and the corpus callosum (4) and in a subtype of cortical interneurons (5).

1 ) Elsir T et al., Cancer Metastasis Rev (2012)
2 ) Lavado A et al., Dev Dyn (2007)
3 ) Lavado A et al., PLoS Biol (2010)
4 ) Bunk EC et al., Stem Cells (2016)
5 ) Rubin AN et al., PLoS One (2013)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Cat. No. 509 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P (FFPE): 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-130C9
Subtype	IgG2a (λ light chain)
Immunogen	Synthetic peptide corresponding to residues near the carboxy terminus of mouse Prox1 (UniProt Id: P48437)
Reactivity	Reacts with: mouse (P48437), rat (A0A8I6A8R7). Other species not tested yet.
Remarks	IHC: For optimal results in retina tissue, follow the retina protocol.
Data sheet	Data Sheet 509_011 Safety Data Sheet (SDS)

Prox1 antibody - 509 011