Synaptosome lysate - 507-P2

Ready to use synaptosome lysate for western blots

Mouse tissue lysate

Cat. No.: 507-P2

Amount: 100 µg

Price: $285.00

Cat. No. 507-P2	100 µg lysate in 1 X SDS PAGE loading buffer 'ready to use'. Total volume: 50 µl. Concentration: 2 mg/ml. Aliquot and store at -20°C until use.
Applications	WB: yes Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Loading/lane	10 µg
Source	Mouse, Balb-C
Remarks	Since several synaptic proteins aggregate after boiling, these lysates are unboiled. Boil sample before loading, if this is recommended for your protocol.
Data sheet	Datasheet 507-p2
Documents	MSDS

Cat. No.: 507-P2

Amount: 100 µg

Price: $285.00

Background

In many cases the relative concentration of the protein of interest is high enough to use whole brain lysate for Western blot testing. If the concentration is less than 0.2% of total protein it may be difficult to distinguish signals from background noise. Since a lot of proteins are localized in distinct cellular compartments, subfractioning may overcome this problem. This fraction enriched for synaptosomes (P2) been prepared according to our protocol. Protein concentration has been determined with a Bradford protein quantification assay.

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