Synaptic Systems - Collagen type IV

Cat. No.: 462 017

Amount: 100 µg

Price: $420.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

IHC
(4 img)

IHC-P (FFPE)
(4 img)

IHC-Fr
(4 img)

Background

Collagen type IV is the most abundant structural component of the basement membrane, a specialized extracellular matrix, that supports epithelial and endothelial cells and separates tissues from their surrounding environments. Collagen type IV consists of polypeptide chains derived from six different alpha chains that assemble into three different collagen type IV isoforms. Unlike fibrillar collagens, collagen IV forms flexible, sheet-like networks that provide structural stability and interact with other basement membrane components, including laminins, nidogens, and proteoglycans (1,2).
The principal functions of collagen IV are to provide mechanical support, maintain basement membrane integrity, regulate cell adhesion, and contribute to tissue organization and cellular signaling. Through its interactions with cell-surface receptors and other extracellular matrix proteins, collagen IV also influences cellular behavior and tissue homeostasis (1).
Dysregulation of collagen IV is associated with several important diseases. Pathogenic variants can cause e.g. diabetic nephropathy, Alport syndrome, and cerebral small-vessel disease as well as increasing the risk of intracerebral hemorrhage and other neurological manifestations (2,3).

1 ) Khoshnoodi J et al., Microsc Res Tech (2008)
2 ) Massoudi D et al., Nat Rev Nephrol (2025)
3 ) Cosgrove D et al., Matrix Biol (2017)

Protocols

IHC

IHC-P (FFPE)

IHC-Fr

Other products for this target

Cat. No. 462 017	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not recommended ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P (FFPE): 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-Fr: 1 : 500 up to 1 : 1000 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-245D2
Subtype	IgG2a (κ light chain)
Immunogen	Human heterotrimeric Collagen type IV containing two α 1-like (UniProt Id: P02462) and one α 2-like (UniProt Id: P08572) chains.
Reactivity	Reacts with: mouse (P02463), mouse (P08122), human (P02462), human (P08572). No signal: rat (F1MA59), rat (F1M6Q3). Other species not tested yet.
Remarks	IHC: One of the following antigen retrievals is required: citrate buffer pH 6, pepsin. IHC-P (FFPE): Antigen retrieval with citrate buffer pH 6, followed by pepsin treatment, is required for chromogenic detection. For chromogenic detection, an optimized AGR time of 30 minutes is recommended for best results. IHC-Fr: The following fixatives are possible: 4% formaldehyde/PFA, methanol, acetone, methanol-acetone.
Data sheet	Data Sheet 462_017 Safety Data Sheet (SDS)

Collagen type IV antibody - 462 017