|Cat. No. 459 005||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 58 to 68 from mouse Protachykinin-1 (UniProt Id: P41539)|
Reacts with: mouse (P41539), rat (P06767).
Other species not tested yet.
|Specificity||The antibody is specific for substance P. It shows no cross-reactivity to neurokinin A, neurokinin B, or the unprocessed precursor protein.|
Substance P (also designated SP or NK-1) is a highly conserved peptide that was originally discovered in 1931 (1). It is an undecapeptide belonging to the tachykinin neuropeptide family. Mammalian tachykinins include substance P, neurokinin A and neurokinin B, and they all share the same carboxy-terminal amino acid sequence (2,3).
Substance P is generated by post-translational cleavage of the precursor Protachykinin-1 (2,3).
Substance P is widely distributed throughout the central and peripheral nervous system, including the brain, spinal cord, and nerve fibers and endocrine cells of the gastrointestinal tract (3,4).
Substance P exerts its effects by binding to the neurokinin-1 receptor NK-1R (2,3).
It functions in the central and peripheral nervous systems as a neurotransmitter or neuromodulator (5) and is involved in many biological processes, including intestinal smooth muscle contraction, blood pressure regulation, neurogenic inflammation, nociception and pain transmission (2,5). Furthermore, it has gained attention for its role in complex psychiatric processes including stress, anxiety, and depression (2).