BCAS1 antibody - 445 111

BCAS1 is a protein specific for an actively myelinating oligodendrocyte subpopulation

Mouse monoclonal purified IgG

Cat. No.: 445 111

Amount: 100 µg

Price: $420.00

Cat. No. 445 111	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 gallery IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 (see remarks) gallery IHC-P (FFPE): 1 : 1000 up to 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-338A5
Immunogen	Synthetic peptide corresponding to residues surrounding (AA 350) of mouse BCAS1 (UniProt Id: Q80YN3)
Reactivity	Reacts with: mouse (Q80YN3), rat (Q3ZB98). No signal: human (O75363-1). Other species not tested yet.
Specificity	Antibody detects mouse BCAS1 isoforms with uniprot IDs Q80YN3 and F7BNZ5. It does not detect isoforms E9Q8Q5 and A2AVX1. It detects rat isoforms Q3ZB98-3, -4, -5 and -5 and -6 and is negative for isoforms -1 and -2.
Remarks	IHC: Antigen retrieval with citrate buffer pH 6 can be applied to improve the signal-to-noise ratio.
Data sheet	Datasheet 445_111

Cat. No.: 445 111

Amount: 100 µg

Price: $420.00

Immunofluorescence image of a FFPE rat cortex section stained for BCAS1 (red) and GFAP (green)

WB
(2 img)

ICC
(2 img)

IHC
(3 img)

IHC-P (FFPE)
(5 img)

Background

Breast carcinoma-amplified sequence 1 (BCAS1) was originally identified as a potential oncogene amplified in human cancer cell lines (1). It is also highly expressed in brain (2) where it mainly defines an oligodendroglial subpopulation occuring at regions of active myelin formation (3).
In fetal brain BCAS1 positive oligodendrocytes are restricted to the white matter. In adult brains they persist in the grey matter until old age (3).

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Collins C et al., Proc. Natl. Acad. Sci. U.S.A. (1998)
2 ) Ishimoto T et al., Glia (2017)
3 ) Fard MK et al., Sci Transl Med (2017)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

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