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BCAS1 antibody - 445 003

BCAS1 is a protein specific for an actively myelinating oligodendrocyte subpopulation
Rabbit polyclonal purified antibody
Cat. No.: 445 003
Amount: 50 µg
Price: $385.00
Cat. No. 445 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 633 from mouse BCAS1 (UniProt Id: Q80YN3)
Reactivity Reacts with: mouse (Q80YN3), rat (Q3ZB98-1), human (O75363).
Other species not tested yet.
Specificity Specific for BCAS1 and its known isoforms
Remarks

IHC: AGR can improve signal strength and reveals additional immunoreactive structures.
Data sheet 445_003.pdf

References for BCAS1 - 445 003

LPCAT1, the Enzyme Responsible for Converting LPC to PC, Promotes OPC Differentiation In Vitro.
Shang Q, Zhang X, Pu Y, Lin J, Ma P, Pan Y, Zhao M, Sun D, Cao L
Journal of cellular and molecular medicine (2025) 293: e70387. 445 003 ICC; tested species: mouse
Oligodendrocyte mechanotransduction channel TMEM63A regulates myelin sheath geometry.
Dereddi RR, Djannatian M, Fiore F, Kalita D, Verkest C, Torres FB, Möbius W, Khodaie B, Ruhwedel T, Alhalaseh K, Schifferer M, et al.
Neuron (2026) : . 445 003 IHC; tested species: mouse
Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome.
Siems SB, Gargareta VI, Schadt LC, Daguano Gastaldi V, Jung RB, Piepkorn L, Casaccia P, Sun T, Jahn O, Werner HB
Glia (2025) 731: 38-56. 445 003 IHC; tested species: mouse
Transcriptional dynamics of the oligodendrocyte lineage and its regulation by the brain erythropoietin system.
Ye L, Daguano Gastaldi V, Curto Y, Wildenburg AF, Yu X, Hindermann M, Eggert S, Ronnenberg A, Wang Q, Butt UJ, Kawaguchi R, et al.
Nature communications (2025) 161: 8291. 445 003 IHC; tested species: mouse
Genetic targeting of premyelinating oligodendrocytes reveals activity-dependent myelination mechanisms.
Bhambri A, Thai P, Wei S, Bae HG, Reynolds P, Barbosa D, Sharma T, Yu Z, Xing C, Kim JH, Yu G, et al.
Nature neuroscience (2025) : . 445 003 IHC; tested species: mouse
Micro-scale control of oligodendrocyte morphology and myelination by the intellectual disability-linked protein acyltransferase ZDHHC9.
Jeong HK, Gonzalez-Fernandez E, Crawley I, Coakley JM, Hwang J, Martin DDO, Bamji SX, Kim JI, Kang SH, Thomas GM
eLife (2025) 13: . 445 003 IHC; tested species: mouse
Oligodendrocytes produce amyloid-β and contribute to plaque formation alongside neurons in Alzheimer's disease model mice.
Sasmita AO, Depp C, Nazarenko T, Sun T, Siems SB, Ong EC, Nkeh YB, Böhler C, Yu X, Bues B, Evangelista L, et al.
Nature neuroscience (2024) : . 445 003 IHC; tested species: mouse
Oligodendrocyte Maturation Alters the Cell Death Mechanisms That Cause Demyelination.
Chapman TW, Kamen Y, Piedra ET, Hill RA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4413: . 445 003 IHC; tested species: mouse
Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
Boutou A, Roufagalas I, Politopoulou K, Tastsoglou S, Abouzeid M, Skoufos G, Verdu de Juan L, Ko JH, Kyrargyri V, Hatzigeorgiou AG, Barnum CJ, et al.
Cell reports (2024) 4311: 114894. 445 003 IHC; tested species: mouse
Transcription Factors Sox8 and Sox10 Contribute with Different Importance to the Maintenance of Mature Oligodendrocytes.
Jörg LM, Schlötzer-Schrehardt U, Lefebvre V, Sock E, Wegner M
International journal of molecular sciences (2024) 2516: . 445 003 IHC; tested species: mouse
Transcription Factors Sox2 and Sox3 Directly Regulate the Expression of Genes Involved in the Onset of Oligodendrocyte Differentiation.
Rupprecht J, Reiprich S, Baroti T, Christoph C, Sock E, Fröb F, Wegner M
Cells (2024) 1311: . 445 003 IHC; tested species: mouse
Cerebral Creatine Deficiency Affects the Timing of Oligodendrocyte Myelination.
Rosko LM, Gentile T, Smith VN, Manavi Z, Melchor GS, Hu J, Shults NV, Albanese C, Lee Y, Rodriguez O, Huang JK, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 437: 1143-1153. 445 003 IHC; tested species: mouse
Oligodendrocyte death initiates synchronous remyelination to restore cortical myelin patterns in mice.
Chapman TW, Olveda GE, Bame X, Pereira E, Hill RA
Nature neuroscience (2023) 264: 555-569. 445 003 IHC; tested species: mouse
PBAF Subunit Pbrm1 Selectively Influences the Transition from Progenitors to Pre-Myelinating Cells during Oligodendrocyte Development.
Waldhauser V, Baroti T, Fröb F, Wegner M
Cells (2023) 1212: . 445 003 IHC; tested species: mouse
Mature and Myelinating Oligodendrocytes Are Specifically Vulnerable to Mild Fluid Percussion Injury in Mice.
Adams AA, Wood TL, Kim HA
Neurotrauma reports (2023) 41: 433-446. 445 003 IHC; tested species: mouse
Cat. No.: 445 003
Amount: 50 µg
Price: $385.00
LPCAT1, the Enzyme Responsible for Converting LPC to PC, Promotes OPC Differentiation In Vitro.
Shang Q, Zhang X, Pu Y, Lin J, Ma P, Pan Y, Zhao M, Sun D, Cao L
Journal of cellular and molecular medicine (2025) 293: e70387. 445 003 ICC; tested species: mouse
Oligodendrocyte mechanotransduction channel TMEM63A regulates myelin sheath geometry.
Dereddi RR, Djannatian M, Fiore F, Kalita D, Verkest C, Torres FB, Möbius W, Khodaie B, Ruhwedel T, Alhalaseh K, Schifferer M, et al.
Neuron (2026) : . 445 003 IHC; tested species: mouse
Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome.
Siems SB, Gargareta VI, Schadt LC, Daguano Gastaldi V, Jung RB, Piepkorn L, Casaccia P, Sun T, Jahn O, Werner HB
Glia (2025) 731: 38-56. 445 003 IHC; tested species: mouse
Transcriptional dynamics of the oligodendrocyte lineage and its regulation by the brain erythropoietin system.
Ye L, Daguano Gastaldi V, Curto Y, Wildenburg AF, Yu X, Hindermann M, Eggert S, Ronnenberg A, Wang Q, Butt UJ, Kawaguchi R, et al.
Nature communications (2025) 161: 8291. 445 003 IHC; tested species: mouse
Genetic targeting of premyelinating oligodendrocytes reveals activity-dependent myelination mechanisms.
Bhambri A, Thai P, Wei S, Bae HG, Reynolds P, Barbosa D, Sharma T, Yu Z, Xing C, Kim JH, Yu G, et al.
Nature neuroscience (2025) : . 445 003 IHC; tested species: mouse
Micro-scale control of oligodendrocyte morphology and myelination by the intellectual disability-linked protein acyltransferase ZDHHC9.
Jeong HK, Gonzalez-Fernandez E, Crawley I, Coakley JM, Hwang J, Martin DDO, Bamji SX, Kim JI, Kang SH, Thomas GM
eLife (2025) 13: . 445 003 IHC; tested species: mouse
Oligodendrocytes produce amyloid-β and contribute to plaque formation alongside neurons in Alzheimer's disease model mice.
Sasmita AO, Depp C, Nazarenko T, Sun T, Siems SB, Ong EC, Nkeh YB, Böhler C, Yu X, Bues B, Evangelista L, et al.
Nature neuroscience (2024) : . 445 003 IHC; tested species: mouse
Oligodendrocyte Maturation Alters the Cell Death Mechanisms That Cause Demyelination.
Chapman TW, Kamen Y, Piedra ET, Hill RA
The Journal of neuroscience : the official journal of the Society for Neuroscience (2024) 4413: . 445 003 IHC; tested species: mouse
Microglia regulate cortical remyelination via TNFR1-dependent phenotypic polarization.
Boutou A, Roufagalas I, Politopoulou K, Tastsoglou S, Abouzeid M, Skoufos G, Verdu de Juan L, Ko JH, Kyrargyri V, Hatzigeorgiou AG, Barnum CJ, et al.
Cell reports (2024) 4311: 114894. 445 003 IHC; tested species: mouse
Transcription Factors Sox8 and Sox10 Contribute with Different Importance to the Maintenance of Mature Oligodendrocytes.
Jörg LM, Schlötzer-Schrehardt U, Lefebvre V, Sock E, Wegner M
International journal of molecular sciences (2024) 2516: . 445 003 IHC; tested species: mouse
Transcription Factors Sox2 and Sox3 Directly Regulate the Expression of Genes Involved in the Onset of Oligodendrocyte Differentiation.
Rupprecht J, Reiprich S, Baroti T, Christoph C, Sock E, Fröb F, Wegner M
Cells (2024) 1311: . 445 003 IHC; tested species: mouse
Cerebral Creatine Deficiency Affects the Timing of Oligodendrocyte Myelination.
Rosko LM, Gentile T, Smith VN, Manavi Z, Melchor GS, Hu J, Shults NV, Albanese C, Lee Y, Rodriguez O, Huang JK, et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 437: 1143-1153. 445 003 IHC; tested species: mouse
Oligodendrocyte death initiates synchronous remyelination to restore cortical myelin patterns in mice.
Chapman TW, Olveda GE, Bame X, Pereira E, Hill RA
Nature neuroscience (2023) 264: 555-569. 445 003 IHC; tested species: mouse
PBAF Subunit Pbrm1 Selectively Influences the Transition from Progenitors to Pre-Myelinating Cells during Oligodendrocyte Development.
Waldhauser V, Baroti T, Fröb F, Wegner M
Cells (2023) 1212: . 445 003 IHC; tested species: mouse
Mature and Myelinating Oligodendrocytes Are Specifically Vulnerable to Mild Fluid Percussion Injury in Mice.
Adams AA, Wood TL, Kim HA
Neurotrauma reports (2023) 41: 433-446. 445 003 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC
IHC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
Background

Breast carcinoma-amplified sequence 1 (BCAS1) was originally identified as a potential oncogene amplified in human cancer cell lines (1). It is also highly expressed in brain (2) where it mainly defines an oligodendroglial subpopulation occuring at regions of active myelin formation (3).
In fetal brain BCAS1 positive oligodendrocytes are restricted to the white matter. In adult brains they persist in the grey matter until old age (3).

 

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.
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