|Cat. No. 444 003||
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: not tested yet
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 2000 Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) is recommended. gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet
|Immunogen||Recombinant protein corresponding to AA 601-629 in mouse TRPV1 (UniProt Id: Q704Y3)|
Reacts with: mouse (Q704Y3).
Weaker signal: rat (O35433).
Other species not tested yet.
TRPV1 (Transient receptor potential cation channel subfamily V member 1, also capsaicin receptor and vanilloid receptor 1) is a member of the TRPV group of the transient receptor potential family of ion channels (1, 3). Expression has been mainly reported in sensory neurons of the peripheral and central nervous system (2, 3). The receptor is activated by a wide variety of exogenous and endogenous physical and chemical stimuli like temperature greater than 42°C; acidic conditions (pH<6); vanilloids, like capsaicin, and allyl isothiocyanate (3). In addition to direct activation, TRPV1 channel activity can also be modulated by inflammatory mediators, such as prostaglandins and bradykinin (4). TRPV1 is involved in the detection and regulation of body temperature, as well as in the sensation of noxious environmental stimuli, like scalding heat and pain. TRPV1 acts as ionotropic endocannabinoid receptor with central neuromodulatory effects and is a prime target for the development of analgesics (5).