Synaptic Systems

Cat. No.: 443 008

Amount: 50 µg

Price: $420.00

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Indirect immunostaining of a rat cortex section for VIP (red) and CCK-8 (green)

ICC
(4 img)

IHC
(9 img)

IHC-P (FFPE)
(3 img)

Background

VIP (Vasoactive intestinal peptide) is a 28 amino acid peptide hormone, a member of the secretin/glucagon superfamily, and evolutionarily well conserved. It is synthesized as part of a larger propeptide which is proteolytically processed to release bioactive VIP as well as two other neuropeptides.

VIP is expressed in gastrointestinal tissues and in the central and peripheral nervous system. It is released from neurons upon depolarization and is known to stimulate adenylyl cyclase.

In the brain, VIP acts as a neurotransmitter and is involved in rhythm generation in the suprachiasmatic nucleus, the regulation of neuroendocrine secretions in the hypothalamus and energy metabolism of glial cells.

Peripherally, VIP influences many functions including blood flow and cardiac output, smooth muscle activity, secretion in the digestive tract, gastric motility, bronchodilation and activity within the hypothalamic-pituitary-adrenal axis.

The Verner-Morrison or Watery Diarrhea Hypokalemia and Achlorhydria (WDHA) syndrome is a characteristic clinical syndrome associated with overproduction of VIP from endocrine tumors.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Iwasaki M et al., F1000Res (2019)
2 ) Deng G et al., Neurol. Res. (2017)
3 ) Moody TW et al., Curr Opin Endocrinol Diabetes Obes (2016)
4 ) Chaudhury D et al., BMC Neurosci (2008)
5 ) Vosko AM et al., Gen. Comp. Endocrinol. ()

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Cat. No. 443 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 100 up to 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb174A6
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to the processed bioactive VIP neuropeptide cleaved from the mouse precursor (UniProt Id: P32648)
Reactivity	Reacts with: mouse (P32648), rat (P01283), human (P01282). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on a monoclonal rat antibody. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	Data Sheet 443_008 Safety Data Sheet (SDS)

VIP antibody - 443 008