beta-Galactosidase antibody - 435 004

beta-Galactosidase is a frequently used reporter protein

Guinea pig polyclonal antiserum

Cat. No.: 435 004

Amount: 100 µl

Price: $370.00

Cat. No. 435 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery ICC: 1 : 500 up to 1 : 1000 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 1024 from E.coli β-galactosidase (UniProt Id: P00722)
Specificity	Specific for β-Galactosidase.
Data sheet	Datasheet 435_004

Cat. No.: 435 004

Amount: 100 µl

Price: $370.00

ICC

IHC

IHC-P

Background

Galactosidases are enzymes that catalyze the hydrolysis of galactosides into monosaccharides. They can be classified as α- or β-Galactosidase. In E.coli the latter one is encoded by the Lac-Z gene and is responsible for breaking down the disaccharide lactose into its monosaccharide components, glucose and galactose.

β-Galactosidase can be used to track the efficiency of bacterial transformation with a recombinant plasmid in a process called Blue/White Color Screening. It is also a frequently used reporter gene to monitor tissue specific gene-expression.

1 ) Bianchi LM et al., J. Histochem. Cytochem. (2002)
2 ) Garcia-Cabrera I et al., Invasion Metastasis (1996)
3 ) Pedersen PH et al., Int. J. Cancer (1995)
4 ) Engström Y et al., Rouxs Arch. Dev. Biol. (1992)
5 ) Boothman DA et al., FEBS Lett. (1989)
6 ) Glaser G et al., Nucleic Acids Res. (1980)
7 ) LESTER G et al., J. Bacteriol. (1952)

Protocols

ICC

Fixed Cells

IHC

IHC-P