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Cat. No. 433 004
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100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
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Applications
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WB: 1 : 1000 up to 1 : 2000 (AP staining) gallery IP: not tested yet ICC: not recommended IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery IHC-P: 1 : 1000 gallery
Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
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Immunogen
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Recombinant protein corresponding to AA 1 to 190 from mouse Dnal1 (UniProt Id: Q05A62)
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Reactivity
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Reacts with: mouse (Q05A62), rat (A0A096MJZ0). Other species not tested yet.
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Remarks
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IHC: Antigen retrieval with citrate buffer pH 6 is required.
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Data sheet
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433_004.pdf
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