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Cat. No. 425 011
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100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
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Applications
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WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) galleryIHC-P (FFPE): 1 : 500 up to 1 : 1000 gallery
Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
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Clone
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35F8
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Subtype
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IgG2a (κ light chain)
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Immunogen
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Recombinant protein corresponding to AA 489 to 614 from rat AChE (UniProt Id: P37136)
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Reactivity
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Reacts with: mouse (P21836), rat (P37136), human (P07101).
Other species not tested yet.
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Specificity
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Specific for AChE, may cross react to isoforms R and H.
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Remarks
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IHC: Antigen retrieval with citrate buffer pH 6 is required.
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Data sheet
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Data Sheet 425_011
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