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Cat. No. 425 006
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200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
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Applications
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WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 (see remarks) galleryIHC-P (FFPE): 1 : 100 up to 1 : 250 (see remarks) gallery
Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
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Immunogen
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Recombinant protein corresponding to AA 489 to 614 from rat AChE (UniProt Id: P37136)
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Reactivity
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Reacts with: rat (P37136), mouse (P21836).
Other species not tested yet.
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Specificity
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Detects isoform T, may cross react to isoforms R and H.
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Remarks
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IHC: Antigen retrieval with citrate buffer pH 6 is required.
IHC-P (FFPE): Antigen retrieval with Tris-EDTA buffer pH 9 is recommended for fluorescent detection.
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Data sheet
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Data Sheet 425_006
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