SENP1 antibody - 422 003

SENP1 is a SUMO specific protease

Rabbit polyclonal purified antibody

Cat. No.: 422 003

Amount: 50 µg

Price: $380.00

Cat. No. 422 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): 1 : 1000 up to 1 : 2000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 623 to 640 from mouse SENP1 (UniProt Id: P59110)
Reactivity	Reacts with: mouse (P59110). Other species not tested yet.
Matching control protein/peptide	422-0P
Data sheet	Datasheet 422_003

Cat. No.: 422 003

Amount: 50 µg

Price: $380.00

IHC-P (FFPE)

Background

The Small ubiquitin-related modifiers SUMOs are ubiquitin-like proteins (UBLs) that can be conjugated to other proteins in a manner analogous to ubiquitin.

Modification of proteins with SUMOs requires a unique activating enzyme complex as well as a conjugation enzyme. An important step is the processing of longer precursors by SUMO-specific proteases, known as SENPs, which remove several C-terminal amino acids of SUMO to expose the Gly-Gly motif.

1 ) Choi JH et al., Neuropharmacology (2016)
2 ) Shao L et al., Nat Commun (2015)
3 ) Girach F et al., Cell Rep (2013)
4 ) Sharma P et al., Cell Rep (2013)

Protocols

IHC-P (FFPE)

Other products for this target