CGRP antibody - 414 017 K.O.

CGRP is a potent peptide vasodilator that can function in the transmission of pain

Rat monoclonal purified IgG

Cat. No.: 414 017

Amount: 100 µg

Price: $420.00

Cat. No. 414 017	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 100 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	SY-310C1
Subtype	IgG2b (κ light chain)
Immunogen	Synthetic peptide corresponding to the C-terminal half of the processed mouse α-CGRP neuropeptide (UniProt Id: Q99JA0)
Reactivity	Reacts with: mouse (Q99JA0), rat (P01256). Other species not tested yet.
Specificity	This antibody recognizes α-CGRP. It may crossreact with the unprocessed precursor protein. K.O. validated
Data sheet	Datasheet 414_017

Cat. No.: 414 017

Amount: 100 µg

Price: $420.00

Indirect immunostaining of a mouse spinal cord section for CGRP (red) and Substance P (green)

IHC
(6 img)

IHC-P (FFPE)
(3 img)

Background

Calcitonin gene-related peptide (CGRP) is a 37-amino acid peptide produced by alternative splicing of calcitonin gene transcripts. It is characterized by a six amino acid ring structure linked by a disulfide bridge and an amidated C-terminus.

CGRP is present in parafollicular cells (C-cells) of the thyroid and in central and peripheral nerves. It is a potent vasodilator and can function in the transmission of pain. It has a major role in migraine pathophysiology, and CGRP antibodies have been used successfully in immunoneutralization.

For more information on protein expression pattern, please refer to the overview image in our SYSY Antibodies ATLAS.

1 ) Palmiter RD et al., Trends Neurosci. (2018)
2 ) Edvinsson L et al., Nat Rev Neurol (2018)
3 ) Benarroch EE et al., Neurology (2011)

Protocols

IHC

IHC-P (FFPE)

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