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Brn3a antibody - 411 017

Brn3a is a transcription factor involved in neuronal development
Rat monoclonal purified IgG
Cat. No.: 411 017
Amount: 100 µg
Price: $415.00
Cat. No. 411 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC-P: 1 : 100 gallery  
Clone SY-61A2
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of mouse Brn3a (UniProt Id: P17208)
Reactivity Reacts with: mouse (P17208), rat (P20266).
Other species not tested yet.

IHC: For optimal results, mild fixation (immersion fixation with 4% PFA for 15 min) according to Gierke et al. 2023 is recommended. Alternatively, heat mediated antigen retrieval (citrate buffer pH 6) can be applied.

Data sheet 411_017.pdf
Cat. No.: 411 017
Amount: 100 µg
Price: $415.00

Brn 3a, also referred to as POU4F1, RGC-1 or Oct-T1, is a transcription factor highly expressed in the developing peripheral sensory nervous system, in cells of the B- and T-lymphocytic lineages and in certain regions of the CNS e.g. retina, spinal cord, midbrain superior colliculus, red nucleus, nucleus ambiguus, inferior olivary nucleus and habenula. In the retina Brn3a is a well-established marker for retinal ganglion cells.