Synaptic Systems - Oxytocin

Cat. No.: 408 008

Amount: 50 µg

Price: $420.00

Indirect immunostaining of a mouse hypothalamus section for Oxytocin (red) and Vasopressin (green)

Dot blot

IHC

IHC-P (FFPE)

Background

Oxytocin is a peptide hormone which is synthesized as an inactive precursor in nerve cell bodies in the supraoptic nucleus and paraventricular nucleus of the hypothalamus. The precursor protein is progressively hydrolyzed to produce oxytocin and its carrier protein neurophysin I. Together with neurophysin I, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream.
Oxytocin is also produced by some neurons of the hypothalamus that project to other parts of the brain and to the spinal cord.
Oxytocin is best known for roles in female reproduction as its release causes smooth muscle contraction during parturition and lactation. It is also involved in cognition, tolerance, adaptation, and complex sexual and maternal behavior.
Oxytocin is used as a medication to facilitate childbirth.

1 ) Baribeau DA et al., Front Neurosci (2015)
2 ) Stoop R et al., Curr. Opin. Neurobiol. (2014)
3 ) Nakajima M et al., Cell (2014)
4 ) Lee HJ et al., Prog. Neurobiol. (2009)

Protocols

IHC

IHC-P (FFPE)

Other products for this target

Cat. No. 408 008	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet Dot blot: 1 : 5000 up to 1 : 20000 (AP staining) (see remarks) gallery IP: not tested yet ICC: not tested yet IHC: 1 : 1000 up to 1 : 5000 gallery IHC-P (FFPE): 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunodetection of target molecules spotted onto a membrane. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb323B2
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to the processed bioactive oxytocin peptide cleaved from mouse oxytocin-neurophysin1 (UniProt Id: P35454)
Reactivity	Reacts with: mouse (P35454), rat (P01179). Other species not tested yet.
Specificity	The antibody preferentially recognizes the processed bioactive oxytocin peptide. The unprocessed precursor protein is only recognized to a limited extent, if at all. The antibody does not crossreact with vasopressin.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody SY-323B2. The constant regions of the heavy and light chains have been replaced with rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. Dot blot: This application was only tested with synthetic peptides.
Data sheet	Datasheet 408_008

Oxytocin antibody - 408 008