Synaptic Systems

Cat. No.: 400 203

Amount: 50 µg

Price: $380.00

Cat. No. 400 203	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 up to 1 : 1000 gallery IHC-P: not recommended Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 189 to 283 from rat TMEM119 (UniProt Id: B2RYL3)
Reactivity	Reacts with: rat (B2RYL3). Weaker signal: mouse (Q8R138). Other species not tested yet.
Data sheet	Datasheet 400_203

References for TMEM119 - 400 203

Microglia regulate neuronal activity via structural remodeling of astrocytes.
Gu N, Makashova O, Laporte C, Chen CQ, Li B, Chevillard PM, Lean G, Yang J, Wong C, Fan J, Sharif B, et al.
Neuron (2025) 11320: 3408-3423.e5. 400 203 IHC; tested species: rat

Significant Sex Differences in the Efficacy of the CSF1R Inhibitor-PLX5622 on Rat Brain Microglia Elimination.
Sharon A, Erez H, Spira ME
Pharmaceuticals (Basel, Switzerland) (2022) 155: . 400 203 IHC; tested species: rat

Inflammatory Foreign Body Response Induced by Neuro-Implants in Rat Cortices Depleted of Resident Microglia by a CSF1R Inhibitor and Its Implications.
Sharon A, Jankowski MM, Shmoel N, Erez H, Spira ME
Frontiers in neuroscience (2021) 15: 646914. 400 203 IHC; tested species: rat

Immunohistological and Ultrastructural Study of the Inflammatory Response to Perforated Polyimide Cortical Implants: Mechanisms Underlying Deterioration of Electrophysiological Recording Quality.
Huang SH, Shmoel N, Jankowski MM, Erez H, Sharon A, Abu-Salah W, Nelken I, Weiss A, Spira ME
Frontiers in neuroscience (2020) 14: 926. 400 203 IHC; tested species: rat

Cat. No.: 400 203

Amount: 50 µg

Price: $380.00

IHC

Background

Microglia are resident myeloid cells of the central nervous system (CNS). They are ontogenetically and functionally distinct from monocyte-derived macrophages that infiltrate the CNS under pathological conditions. Transmembrane protein 119 (TMEM119) is a single-pass type I membrane protein that has been identified as a useful, highly selective microglia marker protein.

1 ) Bennett ML et al., Proc. Natl. Acad. Sci. U.S.A. (2016)
2 ) Satoh J et al., Neuropathology (2016)

Protocols

IHC

Other products for this target

TMEM119 antibody - 400 203

References for TMEM119 - 400 203