Synaptic Systems

Cat. No.: 398 308

Amount: 50 µg

Price: $420.00

Please note that import duties may still apply, which will be billed to you directly by the shipping carrier.

Ki67-positive (red) proliferating intestinal epithelial cells stained with E-cadherin (green) in a mouse colon

ICC
(1 img)

IHC
(3 img)

IHC-P (FFPE)
(6 img)

Background

Ki-67 is a nuclear protein which is widely used as a marker of cell proliferation. Its expression is closely associated with the cell cycle: Ki-67 is present in actively cycling cells during the G1, S, G2, and M phases but is generally absent in quiescent cells in the G0 phase. At the cellular level, Ki-67 is predominantly localized in the nucleus during interphase and redistributes to the periphery of condensed chromosomes during mitosis (1,2).
Immunohistochemical staining for Ki-67 is a simple and reproducible method to determine the tumor proliferative index and is a predictive and prognostic biomarker in certain types of human cancer, such as breast cancer, gastric cancer or prostate cancer. In breast cancer higher Ki-67 levels have been associated with an increased risk of disease recurrence (1,3). However, Ki-67 expression is not specific to malignant cells, as it is also present in normally proliferating tissues and activated lymphocytes.

1 ) Scholzen T et al., J. Cell. Physiol. (2000)
2 ) Sun X et al., Chromosoma (2018)
3 ) de Azambuja E et al., Br J Cancer (2007)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Other products for this target

Cat. No. 398 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: 1 : 1000 up to 1 : 2000 (see remarks) gallery IHC: 1 : 2000 up to 1 : 5000 (see remarks) gallery IHC-P (FFPE): 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	GP311H2
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA 1240 of mouse Ki67 (UniProt Id: E9PVX6)
Reactivity	Reacts with: mouse (E9PVX6). No signal: human (P46013), rat (D4A0Y6). Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone 311H2. The constant regions of the heavy and light chains have been replaced by guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells. ICC: Methanol fixation is recommended. The following fixatives are possible: 4% formaldehyde/PFA. IHC: Antigen retrieval with citrate buffer pH 6 is required. RUO: For this product an additional Limited Use Label License (LULL) for internal Research Use Only (RUO) from Life Technologies Corporation applies. The recombinant proteins/antibodies you will receive/have received from Synaptic Systems GmbH (“Materials“) were provided or produced under a license between Synaptic Systems GmbH and Life Technologies Corporation, a part of Thermo Fisher Scientific, Inc., (“Life Technologies“) through the use of Life Technologies proprietary cell lines and expression products. The only right conveyed to you with respect to the Materials is the limited, non-transferable right to use the Materials to perform internal research for your sole benefit, up to and including pharmacokinetic and toxicology studies required to meet the regulations for filing an IND. IND submissions, first in human research, and/or first in patient research are expressly excluded from the rights provided. No right to resell the Materials, or any portion or component thereof, is conveyed expressly, by implication, or by estoppel. The Materials are not for use in commercial purposes or applications of any kind, including, without limitation, manufacturing or quality control and commercial services such as reporting the results of your activities for a fee or other form of consideration. For information on obtaining additional rights, please contact outlicensing@thermofisher.com or Out Licensing, Life Technologies, 5823 Newton Drive, Carlsbad, California 92008, United States.
Data sheet	Data Sheet 398_308 Safety Data Sheet (SDS)

Ki67 antibody mouse specific - 398 308