Synaptic Systems

Cat. No.: 397 308

Amount: 50 µg

Price: $420.00

F4/80 (red) and IBA1 (green) positive resident Kupffer cells in mouse liver

WB

IHC

IHC-P (FFPE)

Background

The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.

1 ) Gordon S et al., BMC Biol. (2017)
2 ) Gordon S et al., Immunol. Rev. (2014)
3 ) Merad M et al., Annu. Rev. Immunol. (2013)
4 ) Lin HH et al., J. Exp. Med. (2005)
5 ) McKnight AJ et al., J. Leukoc. Biol. (1998)

Protocols

WB

IHC

IHC-P (FFPE)

Other products for this target

Cat. No. 397 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 gallery IP: not tested yet ICC: not tested yet IHC: 1 : 2000 gallery IHC-P (FFPE): 1 : 4000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp167B3
Subtype	IgG2 (κ light chain)
Immunogen	Synthetic peptide corresponding to residues surrounding AA36 of mouse F4/80 (UniProt Id: Q61549)
Reactivity	Reacts with: mouse (Q61549). No signal: rat. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody 167B3. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. The antibody can therefore be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	Datasheet 397_308

F4/80 antibody - 397 308