Taok2 beta antibody - 395 103 K.O.

Taok2 beta is a serine/threonine-protein kinase

Rabbit polyclonal purified antibody

Cat. No.: 395 103

Amount: 50 µg

Price: $380.00

Cat. No. 395 103	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to residues surrounding AA 990 of mouse Taok2 β (UniProt Id: Q6ZQ29-2)
Reactivity	Reacts with: mouse (Q6ZQ29-2), rat (Q9JLS3-2). Other species not tested yet.
Specificity	K.O. validated
Data sheet	Datasheet 395_103

Cat. No.: 395 103

Amount: 50 µg

Price: $380.00

ICC

Background

The serine/threonine-protein kinase Taok 2 or TAO 2 plays a role in membrane blebbing and the formation of apoptotic bodies. Taok 2 is involved in the MAPK14/p38 MAPK stress-activated MAPK cascade. It may also affect the cytoskeletal organization and stability, the dendritic formation, and the osmotic stress-MAPK8 pathway. There are two isoforms, Taok 2α and Taok 2β, only the latter is required for PCDH8 endocytosis and CDH2 cointernalization.

1 ) Yadav S et al., Neuron (2017)
2 ) de Anda FC et al., Nat. Neurosci. (2012)
3 ) Wojtala RL et al., J. Biol. Chem. (2011)
4 ) Chen Z et al., J. Biol. Chem. (2001)
5 ) Chen Z et al., J. Biol. Chem. (1999)

Protocols

ICC

Fixed Cells

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