Synaptic Systems - Orexin A/B

Cat. No.: 389 104

Amount: 100 µl

Price: $370.00

Cat. No. 389 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not tested yet IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 87 to 96 from mouse Orexin (UniProt Id: O55241)
Reactivity	Reacts with: human, rat, mouse (O55241). Other species not tested yet.
Specificity	Recognizes Orexin A and Orexin B with minor cross-reactivity to the unprocessed precursor protein.
Data sheet	Data Sheet 389_104 Safety Data Sheet (SDS)

References for Orexin A/B - 389 104

Still Excited, but Less Aroused-The Effects of Nutritional Ketosis on Epinephrine Response and Hypothalamic Orexin Neuron Activation Following Recurrent Hypoglycemia in Diabetic Rats.
Nedoboy PE, Farnham MM
Metabolites (2022) 131: . 389 104 IHC; tested species: rat

Cat. No.: 389 104

Amount: 100 µl

Price: $370.00

IHC
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IHC-P (FFPE)
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Background

Orexins, also referred to as Hypocretins, are produced by specialized neurons in the lateral hypothalamus, which project to many different regions of the brain. Orexin A (33 amino acids) and Orexin B (28 amino acids) are both produced from a single pre-pro-orexin polypeptide by a cascade of enzymatic reactions. They operate via binding to two closely related G protein-coupled receptors called Orexin receptor 1 and 2 (OxR 1 and OxR 2). OxR 1 is selective for Orexin A whereas OxR 2 binds both peptides with similar affinity.
The orexin system is conserved in mammals, and plays a central role in regulating feeding, sleep/wake cycles, arousal, energy expenditure, reward seeking, cognition, and stress responses. Defects in orexin signaling are involved in diet-induced obesity, diabetes, narcolepsy, panic anxiety disorder, depression, and addiction.

1 ) Huber MJ et al., Cell. Mol. Neurobiol. (2017)
2 ) Villano I et al., Front Behav Neurosci (2017)
3 ) Chow M et al., Nat Sci Sleep (2016)
4 ) Nixon JP et al., Ageing Res. Rev. (2015)
5 ) Hu B et al., Neurosci Biobehav Rev (2015)
6 ) Mahler SV et al., Nat. Neurosci. (2014)
7 ) Perez-Leighton CE et al., Biochim. Biophys. Acta (2014)
8 ) Alexandre C et al., Curr. Opin. Neurobiol. (2013)
9 ) Kodadek T et al., Mol Biosyst (2010)

Protocols

IHC

IHC-P (FFPE)

Orexin A/B antibody - 389 104

References for Orexin A/B - 389 104