Calcineurin A antibody - 387 003

Calcineurin is a calcium/calmodulin dependent Ser/Thr protein phosphatase

Rabbit polyclonal purified antibody

Cat. No.: 387 003

Amount: 50 µg

Price: $460.00

Cat. No. 387 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 2000 up to 1 : 10000 (AP staining) gallery IP: not tested yet ICC: 1 : 1000 up to 1 : 5000 gallery IHC: 1 : 500 up to 1 : 5000 (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 521 from human Calcineurin (UniProt Id: Q08209)
Reactivity	Reacts with: human (Q08209), rat (P63329), mouse (P63328). Other species not tested yet.
Matching control protein/peptide	387-0P
Remarks	IHC: For optimal results in retina tissue, follow the retina protocol.
Data sheet	Datasheet 387_003

Cat. No.: 387 003

Amount: 50 µg

Price: $460.00

ICC

IHC

Background

Calcineurin (PP2B) is a calcium/calmodulin dependent Ser/Thr protein phosphatase that is expressed at high levels in the CNS. It is a heterodimeric protein consisting of a catalytic subunit calcineurin A containing an active site dinuclear metal center, and a tightly associated, myristoylated, Ca²⁺-binding subunit called calcineurin B. Calcineurin is involved in many cellular processes and Ca²⁺-dependent signal transduction pathways.

1 ) Celsi F et al., Neurobiol. Dis. (2007)
2 ) Rusnak F et al., Physiol. Rev. (2000)
3 ) Mulkey RM et al., Nature (1994)
4 ) Liu J et al., Cell (1991)
5 ) Klee CB et al., Proc. Natl. Acad. Sci. U.S.A. (1979)

Protocols

ICC

Fixed Cells

IHC

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