Synaptic Systems - Ankyrin G

Cat. No.: 386 005

Amount: 50 µg

Price: $470.00

Cat. No. 386 005	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 up to 1 : 5000 (AP staining) (see remarks) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery ExM: external data (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to residues near the carboxy terminus of mouse Ankyrin G. (UniProt Id: G5E8K5-1)
Reactivity	Reacts with: rat (O70511-1), mouse (G5E8K5-1). Other species not tested yet.
Specificity	Specific for Ankyrin G, detects all described splice variants. K.O. validated PubMed: 39078369
Remarks	WB: Due to the large size of this protein, we recommend NuPAGE 3-8% Tris-Acetate gels for SDS-PAGE. ExM: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	386_005.pdf

References for Ankyrin G - 386 005

The core PCP protein Prickle2 regulates axon number and AIS maturation by binding to AnkG and modulating microtubule bundling.
Dorrego-Rivas A, Ezan J, Moreau MM, Poirault-Chassac S, Aubailly N, De Neve J, Blanchard C, Castets F, Fréal A, Battefeld A, Sans N, et al.
Science advances (2022) 836: eabo6333. 386 005 WB, IP

Integrative multi-omic analysis reveals conserved cell-projection deficits in human Down syndrome brains.
Rastogi M, Bartolucci M, Nanni M, Aloisio M, Vozzi D, Petretto A, Contestabile A, Cancedda L
Neuron (2024) 11215: 2503-2523.e10. 386 005 ICC; tested species: mouse

An evolutionarily conserved AnkyrinG-dependent motif clusters axonal K2P K+ channels.
Escobedo G, Wu Y, Ogawa Y, Ding X, Rasband MN
The Journal of cell biology (2024) 22310: . 386 005 ICC; KO verified; tested species: mouse,rat

Neurofilament Levels in Dendritic Spines Associate with Synaptic Status.
Gürth CM, do Rego Barros Fernandes Lima MA, Macarrón Palacios V, Cereceda Delgado AR, Hubrich J, D'Este E
Cells (2023) 126: . 386 005 ICC; tested species: rat

Adaptor protein-3 produces synaptic vesicles that release phasic dopamine.
Jain S, Yee AG, Maas J, Gierok S, Xu H, Stansil J, Eriksen J, Nelson AB, Silm K, Ford CP, Edwards RH, et al.
Proceedings of the National Academy of Sciences of the United States of America (2023) 12042: e2309843120. 386 005 ICC; tested species: mouse

Sodium channel endocytosis drives axon initial segment plasticity.
Fréal A, Jamann N, Ten Bos J, Jansen J, Petersen N, Ligthart T, Hoogenraad CC, Kole MHP
Science advances (2023) 937: eadf3885. 386 005 ICC; tested species: mouse

Multilayered regulations of alternative splicing, NMD, and protein stability control temporal induction and tissue-specific expression of TRIM46 during axon formation.
Vuong JK, Ergin V, Chen L, Zheng S
Nature communications (2022) 131: 2081. 386 005 ICC; tested species: mouse

14-3-3 proteins stabilize LGI1-ADAM22 levels to regulate seizure thresholds in mice.
Yokoi N, Fukata Y, Okatsu K, Yamagata A, Liu Y, Sanbo M, Miyazaki Y, Goto T, Abe M, Kassai H, Sakimura K, et al.
Cell reports (2021) 3711: 110107. 386 005 ICC; tested species: mouse

The transcriptional response of cortical neurons to concussion reveals divergent fates after injury.
Alkaslasi MR, Lloyd EYH, Gable AS, Silberberg H, Yarur HE, Tsai VS, Sohn M, Margolin G, Tejeda HA, Le Pichon CE
Nature communications (2025) 161: 1097. 386 005 IHC; tested species: mouse

Axon initial segment dynamics during associative fear learning.
Benoit CM, Ganea DA, Paricio-Montesinos R, Esser J, Thome C, Janssen JM, Sattin A, Innocenti SM, Krabbe S, Lüthi A, Fellin T, et al.
Nature neuroscience (2025) : . 386 005 IHC; tested species: mouse

Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity.
Miyazaki Y, Otsuka T, Yamagata Y, Endo T, Sanbo M, Sano H, Kobayashi K, Inahashi H, Kornau HC, Schmitz D, Prüss H, et al.
Cell reports (2024) 431: 113634. 386 005 IHC; tested species: mouse

The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels.
Ramirez-Franco J, Debreux K, Sangiardi M, Belghazi M, Kim Y, Lee SH, Lévêque C, Seagar M, El Far O
Neurobiology of disease (2024) : 106513. 386 005 IHC; tested species: mouse

Multisensory gamma stimulation mitigates the effects of demyelination induced by cuprizone in male mice.
Rodrigues-Amorim D, Bozzelli PL, Kim T, Liu L, Gibson O, Yang CY, Murdock MH, Galiana-Melendez F, Schatz B, Davison A, Islam MR, et al.
Nature communications (2024) 151: 6744. 386 005 IHC; tested species: mouse

Rescue of Normal Excitability in LGI1-Deficient Epileptic Neurons.
Extrémet J, Ramirez-Franco J, Fronzaroli-Molinieres L, Boumedine-Guignon N, Ankri N, El Far O, Garrido JJ, Debanne D, Russier M
The Journal of neuroscience : the official journal of the Society for Neuroscience (2023) 4350: 8596-8606. 386 005 IHC; tested species: mouse

Light-microscopy-based connectomic reconstruction of mammalian brain tissue.
Tavakoli MR, Lyudchik J, Januszewski M, Vistunou V, Agudelo Dueñas N, Vorlaufer J, Sommer C, Kreuzinger C, Oliveira B, Cenameri A, Novarino G, et al.
Nature (2025) 6428067: 398-410. 386 005 ExM; tested species: mouse

Cat. No.: 386 005

Amount: 50 µg

Price: $470.00

ICC

IHC

IHC-P

Background

Ankyrin G is a membrane-cytoskeleton linker. It may participate in the targeting and clustering of ion channels and cell adhesion molecules at the nodes of Ranvier and axonal initial segments (AIS).

1 ) Fujiwara Y et al., Sci Rep (2016)
2 ) Yang Y et al., J. Cell Biol. (2007)
3 ) Zhou D et al., J. Cell Biol. (1998)
4 ) Wood SJ et al., J. Cell Biol. (1998)
5 ) Kordeli E et al., J. Biol. Chem. (1995)

Protocols

ICC

Fixed Cells

IHC

IHC-P

Standard Condition

Other products for this target

Ankyrin G antibody - 386 005 K.O.

References for Ankyrin G - 386 005