BGT1 antibody - 379 003

BGT1 is a sodium- and chloride-dependent betaine-GABA transporter

Rabbit polyclonal purified antibody

Cat. No.: 379 003

Amount: 50 µg

Price: $380.00

Cat. No. 379 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 567 to 614 from human BGT (UniProt Id: P48065)
Reactivity	Reacts with: human (P48065). Other species not tested yet.
Specificity	Specific for BGT 1
Data sheet	Datasheet 379_003

Cat. No.: 379 003

Amount: 50 µg

Price: $380.00

Background

The sodium- and chloride-dependent betaine-GABA transporter 1 (BGT 1) is a member of the neurotransmitter-sodium-symporter transporter family. It mediates cellular uptake of betaine and GABA in a sodium- and chloride-dependent process.
BGT 1 is involved in betaine transport in the kidney medulla to withstand hyperosmolarity. BGT 1 also transports GABA but its exact role in brain is not yet fully understood.

1 ) Kempson SA et al., Front Physiol (2014)
2 ) Almilaji A et al., Cell. Physiol. Biochem. (2013)
3 ) Kempson SA et al., Cell. Physiol. Biochem. (2013)
4 ) Zhou Y et al., Am. J. Physiol. Renal Physiol. (2012)

Protocols