|Cat. No. 373 003||50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IP: not tested yet
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 100 gallery
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: not tested yet
|Immunogen||Synthetic peptide corresponding to AA 625 to 644 from mouse Numb (UniProt Id: Q9QZS3)|
Reacts with: rat (Q2LC84), mouse (Q9QZS3).
Other species not tested yet.
|Specificity||Specific for Numb. Recognizes all four isoforms. K.O.|
|Matching control protein/peptide||373-0P|
Numb proteins (Numb and Numblike) display a complex pattern of functions such as the control of asymmetric cell division, cell fate choice, endocytosis, cell adhesion, and cell migration. Numb has been shown to inhibit Notch signaling by recruiting α-Adaptin and stimulating endocytosis of Notch. It was also demonstrated that Numb helps activate the tumor suppressor p53, suggesting that loss of Numb in cancerous cells would result in both the activation of the potential oncogene Notch and the diminution of tumor suppression by p53. Numb is itself regulated via ubiquitinylation.
Numb and Numblike are redundant but essential in maintaining neural progenitor cells during early neurogenesis by allowing cells to choose progenitor over neuronal fates. Numb and Numblike were also recently discovered to be involved in cardiac morphogenesis.
Four isoforms of mammalian Numb are described with predicted molecular masses of 65, 66, 71, and 72 kDa.