IgE antibody - 372 011

IgEs are involved in defense against parasites and allergic response

Mouse monoclonal purified IgG

Cat. No.: 372 011

Amount: 100 µg

Price: $420.00

Cat. No. 372 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) (see remarks) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P (FFPE): 1 : 100 gallery ELISA: ; suitable as capture antibody FACS: yes (see remarks) Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Enzyme-linked immunosorbent assay (ELISA); a frequently employed method to quantify target-molecules in solution. The detection of some proteins requires special solubilization steps. For further information, please refer to the „Remarks“ section. Fluorescence-activated cell sorting (FACS); usually cells are fluorescently labeled with antibodies against cell-surface antigens followed by cell-sorter analysis.
Clone	164G11
Subtype	IgG2a (κ light chain)
Immunogen	Purified human basophile granulocytes.
Reactivity	Reacts with: human. Other species not tested yet.
Remarks	WB: Non reducing SDS-PAGE is recommended. To avoid protein aggregation, do not heat samples for SDS-PAGE. FACS: This antibody does not activate basophiles upon binding.
Data sheet	Datasheet 372_011

Cat. No.: 372 011

Amount: 100 µg

Price: $420.00

ICC

IHC-P (FFPE)

Background

Immunglobulin E or IgE is the most rare immunoglobulin class in mammalian serum. IgEs mediate immunity against parasites like helminths and protozoa. They also play a major role in type I hypersensitivity in various allergic diseases.

1 ) Erb KJ et al., Eur. J. Immunol. (2007)
2 ) Gould HJ et al., Annu. Rev. Immunol. (2003)
3 ) Rihet P et al., Eur. J. Immunol. (1991)

Protocols

ICC

Fixed Cells

IHC-P (FFPE)

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