CNP1 (CNPase) antibody - 355 011AT647N K.O.

CNP1 is a marker for oligodendrocytes

Mouse monoclonal purified IgG

Cat. No.: 355 011AT647N

Amount: 100 µg

Price: $470.00

Cat. No. 355 011AT647N	100 µg purified IgG, lyophilized, fluorescence-labeled with ATTO^® 647N. For many of the fluorescence labeled antibodies conjugated to ATTO^® dyes from ATTO-TEC the established fluorescence detection systems can be used. ATTO^® 488: λ_ex 500 nm / λ_em 520 nm ATTO^® 550: λ_ex 554 nm / λ_em 576 nm ATTO^® 565: λ_ex 564 nm / λ_em 590 nm ATTO^® 594: λ_ex 603 nm / λ_em 626 nm ATTO^® 647N: λ_ex 646 nm / λ_em 664 nm ATTO^®488, ATTO^®565, ATTO^®647N and ATTO^®594 dyes are suitable for Stimulated Emission Depletion (STED) microscopy which allows higher resolution imaging compared to confocal laser scanning microscopy. ATTO^®488, ATTO^®565 and ATTO^®594 are also recommended for PALM and dSTORM high resolution microscopy. This product or portions thereof is manufactured under license from ATTO-TEC GmbH. ATTO is a trademarks of ATTO-TEC GmbH, Siegen/Germany. Purchase of reagents related to the AbberiorStar technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from ATTO-TEC GmbH. Albumin was added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	ATTO 647N
Clone	335C6
Subtype	IgG2a (κ light chain)
Immunogen	Full length mouse recombinant CNP1 (UniProt Id: P16330)
Reactivity	Reacts with: rat (P13233), mouse (P16330). Other species not tested yet.
Specificity	K.O. validated
Data sheet	Datasheet 355_011at647n

Cat. No.: 355 011AT647N

Amount: 100 µg

Price: $470.00

ICC

IHC

IHC-P

Background

The 2', 3'-cyclic nucleotide 3'-phosphodiesterase CNP 1, also referred to as CNPase and CNP, is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system.
It is assumed that CNP 1 participates in RNA metabolism of myelinating oligodendrocytes.

1 ) Myllykoski M et al., PLoS ONE (2012)
2 ) Edgar NM et al., Transl Psychiatry (2011)
3 ) Lappe-Siefke C et al., Nat. Genet. (2003)
4 ) Brunner C et al., J. Neurochem. (1989)
5 ) Braun PE et al., J. Neurosci. (1988)

Protocols

ICC

Fixed Cells

IHC

IHC-P

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