CNP1 (CNPase) antibody - 355 011AF647

CNP1 is a marker for oligodendrocytes

Mouse monoclonal purified IgG

Cat. No.: 355 011AF647

Amount: 100 µg

Price: $470.00

Cat. No. 355 011AF647	100 µg purified IgG, lyophilized, fluorescence-labeled with AZdye 647. Fluorescence labeled antibodies conjugated to Alexa^® dyes are well suited for standard epi-fluorescence setups and confocal microscopy. AZdye^® 568: λ_ex 568 nm / λ_em 665 nm (identical to Alexa^® 568) Alexa^® 647: λ_ex 650 nm / λ_em 665 nm This product or portions thereof is manufactured under license from Life Technologies Corporation. Alexa Fluor is a registered trademark of Life Technologies Corporation. Purchase of reagents related to the Alexa Fluor technology from Synaptic Systems GmbH provides a license for non-profit and in-house research use only. Any application of above mentioned technology for commercial purpose requires a separate license from Life Technologies Corporation. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.
Applications	WB: N/A IP: N/A ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Label	AZdye 647
Clone	355C6
Subtype	IgG2a (κ light chain)
Immunogen	Full length mouse recombinant CNP1 (UniProt Id: P16330)
Reactivity	Reacts with: mouse (P16330), rat (P13233). Other species not tested yet.
Data sheet	Datasheet 355_011af647

Cat. No.: 355 011AF647

Amount: 100 µg

Price: $470.00

Immunofluorescence image of rat hippocampus primary cells stained for CNP1 (green) and MAP2 (red)

ICC
(1 img)

IHC
(3 img)

Background

The 2', 3'-cyclic nucleotide 3'-phosphodiesterase CNP1, also referred to as CNPase and CNP, is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system.
It is assumed that CNP1 participates in RNA metabolism of myelinating oligodendrocytes.

1 ) Myllykoski M et al., PLoS ONE (2012)
2 ) Edgar NM et al., Transl Psychiatry (2011)
3 ) Lappe-Siefke C et al., Nat. Genet. (2003)
4 ) Brunner C et al., J. Neurochem. (1989)
5 ) Braun PE et al., J. Neurosci. (1988)

Protocols

ICC

Fixed Cells

IHC

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