Synaptic Systems

Cat. No.: 355 011

Amount: 100 µg

Price: $420.00

Cat. No. 355 011	100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 up to 1 : 5000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	335C6
Subtype	IgG2a (κ light chain)
Immunogen	Full length mouse recombinant CNP1 (UniProt Id: P16330)
Reactivity	Reacts with: rat (P13233), human (P09543), mouse (P16330). Other species not tested yet.
Specificity	K.O. validated
Data sheet	Datasheet 355_011

References for CNP1 - 355 011

Surfactant protein C is associated with perineuronal nets and shows age-dependent changes of brain content and hippocampal deposits in wildtype and 3xTg mice.
Schob S, Puchta J, Winter K, Michalski D, Mages B, Martens H, Emmer A, Hoffmann KT, Gaunitz F, Meinicke A, Krause M, et al.
Journal of chemical neuroanatomy (2021) 118: 102036. 355 011 IHC; tested species: mouse

Immunosignals of Oligodendrocyte Markers and Myelin-Associated Proteins Are Critically Affected after Experimental Stroke in Wild-Type and Alzheimer Modeling Mice of Different Ages.
Michalski D, Keck AL, Grosche J, Martens H, Härtig W
Frontiers in cellular neuroscience (2018) 12: 23. 355 011 IHC; tested species: mouse

Cat. No.: 355 011

Amount: 100 µg

Price: $420.00

WB
(2 img)

ICC
(1 img)

IHC
(5 img)

IHC-P (FFPE)
(4 img)

Background

The 2', 3'-cyclic nucleotide 3'-phosphodiesterase CNP1, also referred to as CNPase and CNP, is one of the most abundant membrane-associated enzymes in the myelin sheath of the vertebrate nervous system.
It is assumed that CNP1 participates in RNA metabolism of myelinating oligodendrocytes.

1 ) Myllykoski M et al., PLoS ONE (2012)
2 ) Edgar NM et al., Transl Psychiatry (2011)
3 ) Lappe-Siefke C et al., Nat. Genet. (2003)
4 ) Brunner C et al., J. Neurochem. (1989)
5 ) Braun PE et al., J. Neurosci. (1988)

Protocols

ICC

Fixed Cells

IHC

IHC-P (FFPE)

Standard Condition

Other products for this target

CNP1 (CNPase) antibody - 355 011 K.O.

References for CNP1 - 355 011