Pantophysin antibody - 354 002

Pantophysin is an ubiquitously expressed synaptophysin relative

Rabbit polyclonal antiserum

Cat. No.: 354 002

Amount: 200 µl

Price: $360.00

Cat. No. 354 002	200 µl antiserum, lyophilized. For reconstitution add 200 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not recommended IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 242 to 261 from mouse Pantophysin (UniProt Id: O09117)
Reactivity	Reacts with: rat (Q66H18), mouse (O09117). Other species not tested yet.
Matching control protein/peptide	354-0P
Data sheet	Datasheet 354_002

Cat. No.: 354 002

Amount: 200 µl

Price: $360.00

ICC

IHC-P (FFPE)

Background

Pantophysin is a transmembrane protein related to the neuroendocrinespecific synaptophysin. It is ubiquitously expressed in all tissues and defines constitutive transport vesicles.

1 ) Brooks CC et al., J. Biol. Chem. (2000)
2 ) Haass NK et al., J. Cell Biol. (1996)

Protocols

ICC

Fixed Cells

IHC-P (FFPE)

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