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Tryptophan hydroxylase 2 - 348 008

An enzyme involved in serotonin synthesis
Monoclonal Recombinant rabbit purified IgG
Cat. No.: 348 008
Amount: 50 µg
Price: $415.00
Cat. No. 348 008 50 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 400 up to 1 : 1000 gallery  
Clone Rb269E7
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 139 from rat Tryptophanhydroxylase 2 (UniProt Id: Q8CGU9)
Reactivity Reacts with: rat (Q8CGU9), mouse (Q8CGV2).
Other species not tested yet.
Specificity Specific for Tryptophan Hydroxylase 2 K.O.
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 269E7. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 348_008.pdf
Cat. No.: 348 008
Quantity: 50 µg
Price: $415.00
Background
Tryptophan hydroxylase (TPH) is an enzyme that catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin).
Two isoforms TPH 1 and TPH 2 have been described. TPH 1 occurs mainly in tissues that express serotonin in the periphery (skin, gut, pineal gland). TPH 2 is exclusively expressed in neuronal cell types and is the predominant isoform in the central nervous system.
In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain.