Synaptic Systems

Cat. No.: 344 004

Amount: 100 µl

Price: $370.00

Jun N-terminal kinase's role in neuropathic pain: C-fiber remodeling and growth-associated protein-43 phosphorylation.
Yamanaka H, Kobayashi K, Kanda H, Noguchi K
Pain reports (2025) 106: e1367. 344 004 IHC; tested species: rat

WB

ICC

IHC

IHC-P

Background

The neuronal growth-associated protein GAP 43 plays an important role in nerve growth and is a major component of the motile "growth cones" that form the tips of elongating axons. It is the major substrate for protein kinase C and is associated with axonal and dendritic filopodia induction. GAP 43 is also known as Neuromodulin, B-50, P-57, pp46, and F1.

1 ) Gnanapavan S et al., J. Neuroimmunol. (2014)
2 ) Donnelly CJ et al., J. Neurosci. (2013)
3 ) Grasselli G et al., PLoS ONE (2011)
4 ) Spencer SA et al., J. Biol. Chem. (1992)
5 ) Rosenthal A et al., EMBO J. (1987)

Protocols

WB

ICC

Fixed Cells

IHC

IHC-P

IP

Standard Condition

Other products for this target

Cat. No. 344 004	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: 1 : 500 gallery IHC-P: 1 : 500 up to 1 : 1000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 1 to 226 from rat GAP43 (UniProt Id: P07936)
Reactivity	Reacts with: rat (P07936), mouse (P06837), human (P17677). Other species not tested yet.
Data sheet	Datasheet 344_004

GAP43 antibody - 344 004

References for GAP43 - 344 004