DAP1 antibody - 342 104

DAP1 is a post-synaptic scaffolding protein

Guinea pig polyclonal antiserum

Cat. No.: 342 104

Amount: 100 µl

Price: $370.00

Cat. No. 342 104	100 µl antiserum, lyophilized. For reconstitution add 100 µl H₂O, then aliquot and store at -20°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 up to 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet ExM: external data (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.
Immunogen	Recombinant protein corresponding to AA 721 to 778 from mouse DAP1 (UniProt Id: Q9D415)
Reactivity	Reacts with: rat (P97836), mouse (Q9D415). Other species not tested yet.
Specificity	Specific for DAP 1; detects all six isoforms known in rat and isoform 1, 2, 3 and 4 in mouse.
Remarks	ExM: This antibody has been successfully applied and published for this method by customers (see application-specific references).
Data sheet	Datasheet 342_104

References for DAP1 - 342 104

Mapping proteomic composition of excitatory postsynaptic sites in the cerebellar cortex.
Robinson K, Delhaye M, Craig AM
Frontiers in molecular neuroscience (2024) 17: 1381534. 342 104 ExM; tested species: mouse

Cat. No.: 342 104

Amount: 100 µl

Price: $370.00

ICC

ExM

Background

Disks large associated proteins (DLGAPs or DAPs) also known as SAP90/PSD-95-associated proteins (SAPAPs) are members of a protein family whose members interact with a membrane-associated guanylate kinase localized at the postsynaptic density (PSD) in neuronal cells. DAPs are adaptor proteins that also interact with other synaptic scaffolding proteins like shanks.

1 ) Welch JM et al., J. Comp. Neurol. (2004)
2 ) Kajimoto Y et al., Neuropsychopharmacology (2003)
3 ) Boeckers TM et al., Biochem. Biophys. Res. Commun. (1999)

Protocols

ICC

Fixed Cells