SERT antibody - 340 308

SERT translocates serotonin back from the synaptic cleft into the presynaptic neuron

Guinea pig monoclonal recombinant IgG

Cat. No.: 340 308

Amount: 50 µg

Price: $420.00

Cat. No. 340 308	50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not recommended IHC: 1 : 500 up to 1 : 2000 gallery IHC-P (FFPE): 1 : 2000 up to 1 : 5000 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Gp64G6
Subtype	IgG2 (κ light chain)
Immunogen	Recombinant protein corresponding to the amino terminal part of mouse SERT (UniProt Id: Q60857)
Reactivity	Reacts with: mouse (Q60857). No signal: human, rat. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal mouse antibody 64G6. The constant regions of the heavy and light chains have been replaced with guinea pig specific sequences. Therefore, the antibody can be used with standard anti-guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	Datasheet 340_308

Cat. No.: 340 308

Amount: 50 µg

Price: $420.00

Indirect immunostaining of a mouse hippocampus section for SERT (red)

IHC

IHC-P (FFPE)

Background

This serotonin transporter (SERT), also referred to as 5HTT and Slc6a4, is a monoamine transporter protein that transports serotonin back from the synaptic cleft into the presynaptic neuron to terminate the action of this neurotransmitter.

1 ) Montgomery TR et al., J. Neurosci. (2014)
2 ) Noristani HN et al., Cell Death Dis (2011)
3 ) Legutko R et al., Brain Res. (2001)
4 ) Najib A et al., FEBS Lett. (2000)
5 ) Fujita M et al., Neurosci. Lett. (1993)
6 ) Langer SZ et al., Experientia (1988)

Protocols

IHC

IHC-P (FFPE)

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