SERT antibody - 340 006

SERT translocates serotonin back from the synaptic cleft into the presynaptic neuron

Chicken polyclonal purified antibody

Cat. No.: 340 006

Amount: 50 µg

Price: $385.00

For the import of chicken antibodies into the USA you need a special import permit. This can be applied for at your USDA office. If you cannot apply for this, please order the chicken antibodies through our distributor Cedarlane USA. If you have any questions, please contact us.

Cat. No. 340 006	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: not recommended IP: not tested yet ICC: not tested yet IHC: 1 : 500 gallery IHC-P (FFPE): 1 : 500 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to the amino terminal part of mouse SERT (UniProt Id: Q60857)
Reactivity	Reacts with: mouse (Q60857). Other species not tested yet.
Data sheet	Datasheet 340_006

Cat. No.: 340 006

Amount: 50 µg

Price: $385.00

IHC

IHC-P (FFPE)

Background

This serotonin transporter (SERT), also referred to as 5HTT and Slc6a4, is a monoamine transporter protein that transports serotonin back from the synaptic cleft into the presynaptic neuron to terminate the action of this neurotransmitter.

1 ) Montgomery TR et al., J. Neurosci. (2014)
2 ) Noristani HN et al., Cell Death Dis (2011)
3 ) Legutko R et al., Brain Res. (2001)
4 ) Najib A et al., FEBS Lett. (2000)
5 ) Fujita M et al., Neurosci. Lett. (1993)
6 ) Langer SZ et al., Experientia (1988)

Protocols

IHC

IHC-P (FFPE)

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