Torsin A antibody - 337 003

TorsinA is a protein involved in DYT1 dystonia

Rabbit polyclonal purified antibody

Cat. No.: 337 003

Amount: 50 µg

Price: $380.00

Cat. No. 337 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not recommended IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 320 to 332 from human TorsinA (UniProt Id: O14656)
Reactivity	Reacts with: human (O14656), rat (Q68G38), mouse (Q9ER39). Other species not tested yet.
Matching control protein/peptide	337-0P
Data sheet	Datasheet 337_003

Cat. No.: 337 003

Amount: 50 µg

Price: $380.00

Background

Torsins are members of the AAA+ superfamily of ATPases which are associated with a variety of cellular activities and are present in all multicellular organisms. They are chaperone-like proteins, involved in protein trafficking, membrane fusion and participating in secretory processing. Torsins are also involved in neurogenesis, neuronal migration, axon/dendrite development and synaptogenesis.
A C-terminal codon deletion in Torsin A, also named Torsin 1A, is associated with inherited DYT1 dystonia.

1 ) Sciamanna G et al., PLoS ONE (2011)
2 ) Kim CE et al., Proc. Natl. Acad. Sci. U.S.A. (2010)
3 ) Yokoi F et al., J. Biochem. (2010)
4 ) Granata A et al., J. Biol. Chem. (2008)
5 ) Hewett J et al., J. Neurosci. Res. (2003)

Protocols

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