Ctip2 antibody C-terminus - 325 003

Ctip2 is a transcription factor involved in neuronal differentiation

Rabbit polyclonal purified antibody

Cat. No.: 325 003

Amount: 50 µg

Price: $380.00

Cat. No. 325 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 gallery IHC: not tested yet IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Recombinant protein corresponding to AA 541 to 812 from rat Ctip2 (UniProt Id: H9N1L3)
Reactivity	Reacts with: rat (H9N1L3), mouse (Q99PV8). Other species not tested yet.
Data sheet	Datasheet 325_003

Cat. No.: 325 003

Amount: 50 µg

Price: $380.00

ICC

Background

The Coup-TFI interacting protein 2 (Ctip 2), also referred to as Bcl 11b, is a zinc finger transcription factor that is expressed in postmitotic neurons.
Together with Satb 2, Coup-TFI, and Fezf2 it is involved in the fine tuned sequential formation and specification of the different excitatory neuron populations forming the definitive six-layered cortical structure.

1 ) Srivatsa S et al., Nat Commun (2014)
2 ) Adiningrat A et al., J. Med. Invest. (2014)
3 ) Cherrier T et al., Proc. Natl. Acad. Sci. U.S.A. (2013)
4 ) Díaz-Alonso J et al., J. Neurosci. (2012)
5 ) Simon R et al., EMBO J. (2012)
6 ) Chen B et al., Proc. Natl. Acad. Sci. U.S.A. (2008)
7 ) Leid M et al., Gene Expr. Patterns (2004)

Protocols

ICC

Fixed Cells

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