Synaptic Systems

Cat. No.: 322 205

Amount: 50 µg

Price: $465.00

Cat. No. 322 205	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: yes ICC: 1 : 500 gallery IHC: external data (see remarks) gallery IHC-P: not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 917 to 940 from rat GABABR2 (UniProt Id: O88871)
Reactivity	Reacts with: rat (O88871), mouse (Q80T41). Other species not tested yet.
Specificity	K.O. validated PubMed: 26691831
Remarks	IHC: This antibody has been successfully applied for this method by our customers using mild fixation (2% PFA at pH 6) according to Lorincz and Nusser 2010 (see gallery). It has not been validated using our standard protocol.
Data sheet	Datasheet 322_205

References for GABABR2 - 322 205

Synaptotagmin-11 facilitates assembly of a presynaptic signaling complex in post-Golgi cargo vesicles.
Trovò L, Kouvaros S, Schwenk J, Fernandez-Fernandez D, Fritzius T, Rem PD, Früh S, Gassmann M, Fakler B, Bischofberger J, Bettler B, et al.
EMBO reports (2024) : . 322 205 WB, ICC; tested species: mouse,human

Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics.
Schwenk J, Pérez-Garci E, Schneider A, Kollewe A, Gauthier-Kemper A, Fritzius T, Raveh A, Dinamarca MC, Hanuschkin A, Bildl W, Klingauf J, et al.
Nature neuroscience (2016) 192: 233-42. 322 205 WB, IP; KO verified; tested species: mouse

Cat. No.: 322 205

Amount: 50 µg

Price: $465.00

ICC

IHC

Background

Metabotropic g-aminobutyric acid type B receptors (GABABRs) are involved in the modulation of synaptic transmission and activity of cerebellar and thalamic neurons.
In contrast to the ionotrophic GABA-A receptors they are linked to G-proteins underlying the slow inhibitory postsynaptic potentials.
GABABR 1 and GABABR 2 have been shown to form heterodimers.

1 ) Kulik A et al., Eur. J. Neurosci. (2002)
2 ) Filippov AK et al., J. Neurosci. (2000)

Protocols

ICC

Fixed Cells

IHC

see remarks

Standard Condition

Other products for this target

GABABR2 antibody - 322 205 K.O.

References for GABABR2 - 322 205