Synaptic Systems

Cat. No.: 320 003

Amount: 50 µg

Price: $380.00

Cat. No. 320 003	50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin was added for stabilization. For reconstitution add 50 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P (FFPE): not tested yet Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen	Synthetic peptide corresponding to AA 185 to 205 from mouse Rab7a (UniProt Id: P51150)
Reactivity	Reacts with: human (P51149), rat (P09527), mouse (P51150). Other species not tested yet.
Matching control protein/peptide	320-0P
Data sheet	Datasheet 320_003

References for Rab7a - 320 003

Regulatory Function of Sympathetic Innervation on the Endo/Lysosomal Trafficking of Acetylcholine Receptor.
Straka T, Schröder C, Roos A, Kollipara L, Sickmann A, Williams MPI, Hafner M, Khan MM, Rudolf R
Frontiers in physiology (2021) 12: 626707. 320 003 WB, IHC; tested species: mouse

Tubular microdomains of Rab7-positive endosomes retrieve TrkA, a mechanism disrupted in Charcot-Marie-Tooth disease 2B.
Markworth R, Dambeck V, Steinbeck LM, Koufali A, Bues B, Dankovich TM, Wichmann C, Burk K
Journal of cell science (2021) 13420: . 320 003 WB, ICC; tested species: mouse

VAPB depletion alters neuritogenesis and phosphoinositide balance in motoneuron-like cells: relevance to VAPB-linked ALS.
Genevini P, Colombo MN, Venditti R, Marcuzzo S, Colombo SF, Bernasconi P, De Matteis MA, Borgese N, Navone F
Journal of cell science (2019) : . 320 003 WB; tested species: mouse

EndophilinAs regulate endosomal sorting of BDNF-TrkB to mediate survival signaling in hippocampal neurons.
Burk K, Murdoch JD, Freytag S, Koenig M, Bharat V, Markworth R, Burkhardt S, Fischer A, Dean C
Scientific reports (2017) 71: 2149. 320 003 ICC; tested species: mouse

Cat. No.: 320 003

Amount: 50 µg

Price: $380.00

Background

Rab 7 is a member of the Rab protein family that belongs to the ras-related superfamily of small monomeric GTPases. Rab 7 is ubiquitously expressed in different tissues where it controls membrane trafficking from early to late endosome and to lysosomes. Rab 7 is also involved in the maturation of phagosome and autophagic vacuoles.

1 ) Girard E et al., Traffic (2014)
2 ) Kawakami A et al., J. Invest. Dermatol. (2008)
3 ) Feng Y et al., J. Cell Biol. (1995)
4 ) Méresse S et al., J. Cell. Sci. (1995)
5 ) Soldati T et al., J. Biol. Chem. (1995)

Protocols

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Rab7a antibody - 320 003

References for Rab7a - 320 003