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Tau antibody - 314 011

Tau is a neuronal microtubule associated protein
Mouse monoclonal purified IgG
Cat. No.: 314 011
Amount: 100 µg
Price: $415.00
Cat. No. 314 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 200 up to 1 : 500 gallery  
IHC: 1 : 100 gallery  
IHC-P: 1 : 500 gallery  
Clone 248E5
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)
Reactivity Reacts with: rat (P19332), mouse (P10637).
Weaker signal: human (P10636).
No signal: zebrafish.
Other species not tested yet.
Specificity This antibody binds phosphorylated and non-phosphorylated tau proteins. The sequence used for immunization is present in all splice variants except human TauA (UniProt Id: P10636-3)
Matching control protein/peptide 314-0P
Remarks

For human tissue cat.no. 314 012 and 314 111 are highly recommended.
WB: Detects the mouse protein with much greater sensitivity than the rat protein.
The antibody binds phosphorylated and non-phosphorylated tau proteins.

Data sheet 314_011.pdf

References for Tau - 314 011

Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 314 011 ICC; tested species: mouse
Postnatal expression profiles of atypical cadherin FAT1 suggest its role in autism.
Frei JA, Brandenburg C, Nestor JE, Hodzic DM, Plachez C, McNeill H, Dykxhoorn DM, Nestor MW, Blatt GJ, Lin YC
Biology open (2021) : . 314 011 ICC; tested species: mouse
Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Gladkov A, Pigareva Y, Kutyina D, Kolpakov V, Bukatin A, Mukhina I, Kazantsev V, Pimashkin A
Scientific reports (2017) 71: 15625. 314 011 ICC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 314 011 ICC
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 314 011 ICC; tested species: mouse
Functional neuronal cells generated by human parthenogenetic stem cells.
Ahmad R, Wolber W, Eckardt S, Koch P, Schmitt J, Semechkin R, Geis C, Heckmann M, Brüstle O, McLaughlin JK, Sirén AL, et al.
PloS one (2012) 78: e42800. 314 011 ICC
Cat. No.: 314 011
Amount: 100 µg
Price: $415.00
Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro.
Teng Z, Gottmann K
Frontiers in molecular neuroscience (2022) 15: 829506. 314 011 ICC; tested species: mouse
Postnatal expression profiles of atypical cadherin FAT1 suggest its role in autism.
Frei JA, Brandenburg C, Nestor JE, Hodzic DM, Plachez C, McNeill H, Dykxhoorn DM, Nestor MW, Blatt GJ, Lin YC
Biology open (2021) : . 314 011 ICC; tested species: mouse
Design of Cultured Neuron Networks in vitro with Predefined Connectivity Using Asymmetric Microfluidic Channels.
Gladkov A, Pigareva Y, Kutyina D, Kolpakov V, Bukatin A, Mukhina I, Kazantsev V, Pimashkin A
Scientific reports (2017) 71: 15625. 314 011 ICC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 314 011 ICC
Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury.
Gut IM, Beske PH, Hubbard KS, Lyman ME, Hamilton TA, McNutt PM
PloS one (2013) 85: e64423. 314 011 ICC; tested species: mouse
Functional neuronal cells generated by human parthenogenetic stem cells.
Ahmad R, Wolber W, Eckardt S, Koch P, Schmitt J, Semechkin R, Geis C, Heckmann M, Brüstle O, McLaughlin JK, Sirén AL, et al.
PloS one (2012) 78: e42800. 314 011 ICC
Background

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.