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Tau antibody - 314 008

Tau is a neuronal microtubule associated protein
Rabbit monoclonal recombinant IgG
Cat. No.: 314 008
Amount: 50 µg
Price: $415.00
Cat. No. 314 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 4000 up to 1 : 20000 gallery  
DNA-PAINT: yes (see remarks)
Clone Rb248E5
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)
Reactivity Reacts with: rat (P19332), mouse (P10637).
Weaker signal: human (P10636).
No signal: zebrafish.
Other species not tested yet.
Specificity This antibody binds phosphorylated and non-phosphorylated tau proteins. The sequence used for immunization is present in all splice variants except human TauA (UniProt Id: P10636-3).
Matching control protein/peptide 314-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 248E5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
DNA-PAINT: This antibody has been successfully used for DNA-PAINT application (see Unterauer et al., 2024; PMID: 38552614).

Data sheet 314_008.pdf

References for Tau - 314 008

Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 314 008 DNA-PAINT; tested species: rat
Cat. No.: 314 008
Amount: 50 µg
Price: $415.00
Spatial proteomics in neurons at single-protein resolution.
Unterauer EM, Shetab Boushehri S, Jevdokimenko K, Masullo LA, Ganji M, Sograte-Idrissi S, Kowalewski R, Strauss S, Reinhardt SCM, Perovic A, Marr C, et al.
Cell (2024) 1877: 1785-1800.e16. 314 008 DNA-PAINT; tested species: rat
Background

There are two major classes of heat stable microtubule associated proteins (MAPS): MAP 2 (280 kD), and tau (55-65 kD). Both protein classes are involved in the regulation of microtubule polymerization in cells. Tau is a neuronal protein that mainly localizes to axons. Hyperphosphorylated tau has been shown to be a major element of paired helical filaments in Alzheimer's disease.