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Cortactin - 313 111

A cortical actin binding protein
Monoclonal mouse purified IgG
Cat. No.: 313 111
Amount: 100 µg
Price: $415.00
Cat. No. 313 111 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: not recommended
IP: not tested yet
ICC: yes (see remarks) gallery  
IHC: not tested yet
IHC-P/FFPE: not tested yet
ELISA: yes
Clone 289H10
Subtype IgG1 (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 513 from human Cortactin (UniProt Id: Q14247)
Epitop Epitop: AA 147 to 509 from human Cortactin (UniProt Id: Q14247)
Reactivity Reacts with: human (Q14247), mouse (Q60598).
Other species not tested yet.
Specificity Specific for cortactin.
Remarks

ICC: Works on PFA and methanol fixed cells, but methanol fixation produces less background.

Data sheet 313_111.pdf

References for Cortactin - 313 111

Cortactin promotes migration and platelet-derived growth factor-induced actin reorganization by signaling to Rho-GTPases.
Lai FP, Szczodrak M, Oelkers JM, Ladwein M, Acconcia F, Benesch S, Auinger S, Faix J, Small JV, Polo S, Stradal TE, et al.
Molecular biology of the cell (2009) 2014: 3209-23. 313 111 ICC
Cat. No.: 313 111
Quantity: 100 µg
Price: $415.00
Cortactin promotes migration and platelet-derived growth factor-induced actin reorganization by signaling to Rho-GTPases.
Lai FP, Szczodrak M, Oelkers JM, Ladwein M, Acconcia F, Benesch S, Auinger S, Faix J, Small JV, Polo S, Stradal TE, et al.
Molecular biology of the cell (2009) 2014: 3209-23. 313 111 ICC
Background

Cortactin is a cortical actin binding protein and the major substrate for the tyrosine kinase v-Src. The interaction of its N-terminal acidic domain (NTA) with the actin nucleation factor Arp 2/3 links cytoskeletal organization with signal transduction. The carboxy terminus comprises a proline rich region and an SH3 domain that can interact with several scaffolding proteins like CortBP1 and Shank 3. In neurons cortactin is concentrated in dendritic spines and involved in spine targeting.