|Cat. No. 313 111||100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.|
WB: not recommended
IP: not tested yet
ICC: yes (see remarks) gallery
IHC: not tested yet
IHC-P/FFPE: not tested yet
|Subtype||IgG1 (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 513 from human Cortactin (UniProt Id: Q14247)|
Epitop: AA 147 to 509 from human Cortactin (UniProt Id: Q14247)
Reacts with: human (Q14247), mouse (Q60598).
Other species not tested yet.
|Specificity||Specific for cortactin.|
ICC: Works on PFA and methanol fixed cells, but methanol fixation produces less background.
Cortactin is a cortical actin binding protein and the major substrate for the tyrosine kinase v-Src. The interaction of its N-terminal acidic domain (NTA) with the actin nucleation factor Arp 2/3 links cytoskeletal organization with signal transduction. The carboxy terminus comprises a proline rich region and an SH3 domain that can interact with several scaffolding proteins like CortBP1 and Shank 3. In neurons cortactin is concentrated in dendritic spines and involved in spine targeting.